Tagliapietra Valentina, Boniotti Maria Beatrice, Mangeli Anna, Karaman Iyad, Alborali Giovanni, Chiari Mario, D'Incau Mario, Zanoni Mariagrazia, Rizzoli Annapaola, Pacciarini Maria Lodovica
Departemt of Biodiversity and Molecular Ecology, Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, 38098 San Michele all'Adige, Italy.
National Reference Centre of Bovine Tuberculosis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy.
Microorganisms. 2021 Oct 2;9(10):2084. doi: 10.3390/microorganisms9102084.
An unexpected high presence of in wild boar in Northern Italy (Garda Lake) has been reported since 2003, but the factors contributing to the maintenance of this pathogen are still unclear. In this study, we investigated the presence of in wild rodents and in water and soil samples collected at wild boar aggregation areas, such as watering holes, with the aim of clarifying their role in transmission. In total, 8 out of 120 captured animals tested positive for the complex (MTBC) as assessed by real-time PCR, and six samples were confirmed to be . A strain with a genetic profile similar to those previously isolated in wild boars in the same area was isolated from one sample. Of the 20 water and 19 mud samples, 3 and 1, respectively, tested positive for the presence of MTBC, and spacer oligotype SB0118 (vole type) was detected in one sample. Our study suggests that wild rodents, in particular , sp. and , play roles in the maintenance of infections in wild boar through ingestion or by contact with either infected excreta or a contaminated environment, such as at animal aggregation sites.
自2003年以来，意大利北部（加尔达湖）野猪体内出现了意外的高含量[此处原文有缺失内容]，但维持这种病原体的因素仍不清楚。在本研究中，我们调查了野生啮齿动物以及在野猪聚集区（如饮水孔）采集的水和土壤样本中[此处原文有缺失内容]的存在情况，目的是阐明它们在[此处原文有缺失内容]传播中的作用。通过实时PCR评估，在120只捕获动物中，共有8只对[此处原文有缺失内容]复合体（MTBC）检测呈阳性，6个样本被确认为[此处原文有缺失内容]。从一个样本中分离出一种遗传特征与之前在同一地区野猪中分离出的菌株相似的菌株。在20份水样本和19份泥浆样本中，分别有3份和1份MTBC检测呈阳性，在一个样本中检测到间隔寡核苷酸型SB0118（田鼠型）。我们的研究表明，野生啮齿动物，特别是[此处原文有缺失内容]属、[此处原文有缺失内容]属和[此处原文有缺失内容]属，通过摄入或接触受感染的排泄物或受污染的环境（如动物聚集场所），在维持野猪的[此处原文有缺失内容]感染中发挥作用。
