Eifan Saleh, Hanif Atif, Nour Islam, Alqahtani Sultan, Eisa Zaki M, Dafalla Ommer, Kohl Alain
Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Saudi Center for Disease Control and Prevention (SCDC), Jazan 82722-2476, Saudi Arabia.
Pathogens. 2021 Oct 15;10(10):1334. doi: 10.3390/pathogens10101334.
Entomologic investigations were conducted in the Al-Darb, Al-Reath, Al-Aridah, Abuareesh, Al-Ahad, Samttah, Sabyah, Damad and Beash areas by CO-baited CDC miniature light traps in the Jazan region. Vectors were identified morphologically, as well as COI gene segment amplification and sequencing. The relative abundance (RA%) and pattern of occurrence (C%) were recorded. The presence of the Rift Valley fever virus (RVFV) in pooled mosquito samples was investigated by reverse transcriptase-polymerase chain reaction (RT-PCR). () and () were found with RA% values of 96% and 4%, respectively, in the region. Significant variations in vector population densities were observed in different districts. The was found highly abundant in all districts and RA% value (100%) was recorded in the Al-Darb, Al-Reath, Al-Aridah, Samttah and Damad areas, whereas RA% values (93.75%, 93.33%, 92.30% and 91.66%) were noted in Al-Ahad, Sabyah, Abuareesh and Beash districts, respectively. RA% values for were recorded as 8.33%, 7.70%, 6.66% and 6.25% in Beash, Abuareesh, Sabyah and Al-Ahad areas, respectively. The pattern of occurrence for and was recorded as 100% and 44.4% in the region. Phylogenetic analysis of and exhibited a close relationship with mosquitoes from Kenya and Turkey, respectively. All mosquito samples tested by RT-PCR were found negative for RVFV. In summary, the current study assessed the composition, abundance, distribution of different mosquito vectors and presence of RVFV in different areas of the Jazan region. Our data will help risk assessments of RVFV future re-emergence in the region.
在吉赞地区，通过用一氧化碳诱饵的疾病控制中心（CDC）微型诱蚊灯在达尔卜、赖斯、阿里达、阿布阿里什、艾哈德、萨姆塔、萨比亚、达马德和贝什等地区开展了昆虫学调查。通过形态学鉴定、细胞色素氧化酶亚基I（COI）基因片段扩增和测序来识别病媒。记录了相对丰度（RA%）和出现模式（C%）。通过逆转录聚合酶链反应（RT-PCR）研究了混合蚊虫样本中裂谷热病毒（RVFV）的存在情况。在该地区发现（某病媒）和（另一病媒）的相对丰度值分别为96%和4%。在不同地区观察到病媒种群密度存在显著差异。（某病媒）在所有地区都大量存在，在达尔卜、赖斯、阿里达、萨姆塔和达马德地区记录到的相对丰度值为100%，而在艾哈德、萨比亚、阿布阿里什和贝什地区记录到的相对丰度值分别为93.75%、93.33%、92.30%和91.66%。在贝什、阿布阿里什、萨比亚和艾哈德地区记录到的（另一病媒）的相对丰度值分别为8.33%、7.70%、6.66%和6.25%。在该地区记录到（某病媒）和（另一病媒）的出现模式分别为100%和44.4%。对（某病媒）和（另一病媒）的系统发育分析表明，它们分别与来自肯尼亚和土耳其的蚊子关系密切。通过RT-PCR检测的所有蚊虫样本均未检测到RVFV呈阳性。总之，本研究评估了吉赞地区不同病媒蚊虫的组成、丰度、分布以及RVFV的存在情况。我们的数据将有助于对该地区未来RVFV再次出现进行风险评估。
