肌醇及其对斑马鱼发育影响的潜在保肝作用研究。
Study of the Potential Hepatoprotective Effect of Myo-Inositol and Its Influence on Zebrafish Development.
机构信息
Department of Human Physiology and Pathophysiology, School of Medicine, Collegium Medicum, University of Warmia and Mazury, 10-082 Olsztyn, Poland.
Students' Scientific Club of Pathophysiologists, Department of Human Physiology and Pathophysiology, School of Medicine, University of Warmia and Mazury, 10-082 Olsztyn, Poland.
出版信息
Nutrients. 2021 Sep 24;13(10):3346. doi: 10.3390/nu13103346.
Inositol is a natural substance found widely in plants. It is used in therapies for many medical cases. The aim of this study was to determine the toxicity of myo-inositol (MI) and to investigate its potential hepatoprotective character. In the first part of the study, zebrafish embryos were incubated with 5, 10, 20, 40, 60, 80, and 100 mg/mL MI. Endpoints such as survivability, hatching rate, malformation, and mobility were evaluated. Our results demonstrated that the high doses of MI lead to increased mortality and malformations and reduce the hatching rate in comparison to the control group. Moreover, low doses of this compound do not produce a negative effect on zebrafish and even have the ability to increase the hatching rate and mobility. In the second part of the study, the hepatoprotective effect of MI was tested. Zebrafish larvae from the line Tg (fabp10a:DsRed) were incubated for 24 h with 1% and 2% ethanol (EtOH), 5 mg/mL of MI with 1% EtOH, and 5 mg/mL of MI with 2% EtOH. No significant differences between the groups with EtOH and the group treated with EtOH with MI were observed. Our results suggest that MI has no positive benefits on hepatocytes of zebrafish larvae.
肌醇是一种广泛存在于植物中的天然物质。它被用于许多医学病例的治疗。本研究的目的是确定肌醇（MI）的毒性，并研究其潜在的肝保护特性。在研究的第一部分，将斑马鱼胚胎在 5、10、20、40、60、80 和 100 mg/mL MI 中孵育。评估了存活率、孵化率、畸形和运动等终点。我们的结果表明，与对照组相比，MI 的高剂量会导致死亡率和畸形增加，并降低孵化率。此外，这种化合物的低剂量不会对斑马鱼产生负面影响，甚至有能力提高孵化率和运动能力。在研究的第二部分，测试了 MI 的肝保护作用。用 1%和 2%乙醇（EtOH）、5 mg/mL MI 加 1%EtOH 和 5 mg/mL MI 加 2%EtOH 孵育 Tg(fabp10a:DsRed)系的斑马鱼幼虫 24 小时。未观察到 EtOH 组与 EtOH 加 MI 组之间有显著差异。我们的结果表明，MI 对斑马鱼幼虫肝细胞没有积极益处。
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