Department of Human Physiology and Pathophysiology, School of Medicine, Collegium Medicum, University of Warmia and Mazury, 10-082 Olsztyn, Poland.
Students' Scientific Club of Pathophysiologists, Department of Human Physiology and Pathophysiology, School of Medicine, University of Warmia and Mazury, 10-082 Olsztyn, Poland.
Nutrients. 2024 Jun 23;16(13):1997. doi: 10.3390/nu16131997.
Myo-inositol (MI) is the most abundant inositol found in nature. To date MI supplementation is reported to be effective in the treatment of polycystic ovary syndrome, it is also suggested to alleviate the symptoms of diabetes and neurodegenerative disorders, but to date no statistically significant effects of inositol on depressive and anxiety symptoms were proven. In the study of anxiolytic effects in zebrafish, we often use the thigmotaxis index measuring the ratio of the amount of time the animal spends near the walls compared to the entire arena.
The objective of this paper was to examine the effect of MI on zebrafish embryos' locomotor activity, as well as its potential anxiolytic activity in zebrafish larvae.
In the first part of the experiment, the embryos were incubated with 5, 10, 20, and 40 mg/mL MI. 1-day post fertilization, embryo mobility was evaluated and burst activity was calculated. In the next part of the study, the behavior of 5-day-old larvae was tested.
Tests on embryo movement showed an increase in burst activity in the MI group at concentrations of 40 mg/mL ( < 0.0001) and a slight decrease in the group at concentrations of 10 mg/mL ( < 0.05). MI in the light/dark challenge had no impact on the thigmotaxis index.
MI was shown to not affect stress reduction in zebrafish larvae. Further research on the potential of MI and other stereoisomers is needed.
肌醇（MI）是自然界中含量最丰富的肌醇。迄今为止，MI 补充剂已被报道可有效治疗多囊卵巢综合征，也可缓解糖尿病和神经退行性疾病的症状，但迄今为止，尚无关于肌醇对抑郁和焦虑症状有统计学意义影响的证据。在研究抗焦虑作用的斑马鱼中，我们经常使用趋触性指数来衡量动物在靠近墙壁的时间与整个竞技场的时间的比例。
本文旨在研究 MI 对斑马鱼胚胎运动活性的影响，以及其对斑马鱼幼虫潜在的抗焦虑活性。
在实验的第一部分，将胚胎与 5、10、20 和 40mg/ml 的 MI 孵育。受精后 1 天，评估胚胎的运动能力并计算爆发活动。在研究的下一部分，测试了 5 日龄幼虫的行为。
对胚胎运动的测试表明，在浓度为 40mg/ml 的 MI 组中，爆发活性增加（<0.0001），在浓度为 10mg/ml 的 MI 组中，爆发活性略有下降（<0.05）。MI 在明暗挑战中对趋触性指数没有影响。
MI 对减少斑马鱼幼虫的应激没有影响。需要进一步研究 MI 和其他立体异构体的潜在作用。
