Abu Bakar Sajak Azliana, Azlan Azrina, Abas Faridah, Hamzah Hazilawati
Department of Nutrition, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia.
Research Centre for Excellence for Nutrition and Non-Communicable Disease, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia.
Nutrients. 2021 Oct 12;13(10):3573. doi: 10.3390/nu13103573.
An herbal mixture composed of lemon, apple cider, garlic, ginger and honey as a polyphenol-rich mixture (PRM) has been reported to contain hypolipidemic activity on human subjects and hyperlipidemic rats. However, the therapeutic effects of PRM on metabolites are not clearly understood. Therefore, this study aimed to provide new information on the causal impact of PRM on the endogenous metabolites, pathways and serum biochemistry. Serum samples of hyperlipidemic rats treated with PRM were subjected to biochemistry (lipid and liver profile) and hydroxymethylglutaryl-CoA enzyme reductase (HMG-CoA reductase) analyses. In contrast, the urine samples were subjected to urine metabolomics using H NMR. The serum biochemistry revealed that PRM at 500 mg/kg (PRM-H) managed to lower the total cholesterol level and low-density lipoprotein (LDL-C) ( < 0.05) and reduce the HMG-CoA reductase activity. The pathway analysis from urine metabolomics reveals that PRM-H altered 17 pathways, with the TCA cycle having the highest impact (0.26). Results also showed the relationship between the serum biochemistry of LDL-C and HMG-CoA reductase and urine metabolites (trimethylamine--oxide, dimethylglycine, allantoin and succinate). The study's findings demonstrated the potential of PRM at 500 mg/kg as an anti-hyperlipidemic by altering the TCA cycle, inhibiting HMG-CoA reductase and lowering the LDL-C in high cholesterol rats.
据报道，一种由柠檬、苹果醋、大蒜、生姜和蜂蜜组成的草药混合物作为富含多酚的混合物（PRM），对人体受试者和高脂血症大鼠具有降血脂活性。然而，PRM对代谢物的治疗效果尚不清楚。因此，本研究旨在提供关于PRM对内源性代谢物、代谢途径和血清生物化学的因果影响的新信息。对用PRM治疗的高脂血症大鼠的血清样本进行生物化学（血脂和肝功能）和羟甲基戊二酰辅酶A还原酶（HMG-CoA还原酶）分析。相比之下，对尿液样本使用核磁共振氢谱进行尿液代谢组学分析。血清生物化学分析表明，500mg/kg的PRM（PRM-H）能够降低总胆固醇水平和低密度脂蛋白（LDL-C）（P<0.05），并降低HMG-CoA还原酶活性。尿液代谢组学的通路分析表明，PRM-H改变了17条通路，其中三羧酸循环的影响最大（0.26）。结果还显示了LDL-C的血清生物化学与HMG-CoA还原酶和尿液代谢物（氧化三甲胺、二甲基甘氨酸、尿囊素和琥珀酸）之间的关系。该研究结果表明，500mg/kg的PRM通过改变三羧酸循环、抑制HMG-CoA还原酶和降低高胆固醇大鼠的LDL-C，具有抗高脂血症的潜力。
