Mayfield S P, Rahire M, Frank G, Zuber H, Rochaix J D
Proc Natl Acad Sci U S A. 1987 Feb;84(3):749-53. doi: 10.1073/pnas.84.3.749.
We have cloned a cDNA encoding a 20-kDa polypeptide, oxygen-evolving enhancer protein 2 (OEE2), in Chlamydomonas reinhardtii. This polypeptide has been implicated in photosynthetic oxygen evolution, and it is associated with the photosystem II complex, the site of oxygen evolution in all higher plants and algae. The sequence of OEE2 cDNA, the deduced amino acid sequence of the preprotein, the N-terminal protein sequence of mature OEE2 protein, and the coding regions of the single OEE2 gene are presented. The protein is synthesized with a 57-amino acid N-terminal transit peptide that directs the transport of this polypeptide across three cellular membranes. A nuclear mutant of C. reinhardtii, deficient in oxygen-evolving activity, is shown to be specifically missing OEE2 polypeptides. This mutation, which results in the complete absence of all OEE2 mRNA and protein, does not affect the accumulation of other photosystem II polypeptides or their mRNAs.
我们已经克隆了莱茵衣藻中一个编码20 kDa多肽（放氧增强蛋白2，OEE2）的cDNA。该多肽与光合放氧有关，并且与光系统II复合物相关，而光系统II复合物是所有高等植物和藻类中放氧的部位。本文给出了OEE2 cDNA的序列、前体蛋白推导的氨基酸序列、成熟OEE2蛋白的N端蛋白序列以及单个OEE2基因的编码区。该蛋白合成时带有一个57个氨基酸的N端转运肽，该转运肽指导此多肽穿过三层细胞膜进行转运。莱茵衣藻的一个缺乏放氧活性的核突变体被证明特异性缺失OEE2多肽。此突变导致所有OEE2 mRNA和蛋白完全缺失，但不影响其他光系统II多肽或其mRNA的积累。
