Salleh Mohd Shahrul Nizam, Ali Roshafima Rasit, Shameli Kamyar, Hamzah Mohd Yusof, Kasmani Rafiziana Md, Nasef Mohamed Mahmoud
School of Chemical Engineering, College of Engineering, Universiti Teknologi MARA Cawangan Terengganu, Bukit Besi Campus, Dungun 23200, Terengganu, Malaysia.
Chemical Process Engineering Department, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Ahmad Petra, Kuala Lumpur 54100, Johor, Malaysia.
Polymers (Basel). 2021 Oct 17;13(20):3578. doi: 10.3390/polym13203578.
The production of pure silver nanoparticles (Ag-NPs) with unique properties remains a challenge even today. In the present study, the synthesis of silver nanoparticles (Ag-NPs) from natural pullulan (PL) was carried out using a radiation-induced method. It is known that pullulan is regarded as a microbial polysaccharide, which renders it suitable to act as a reducing and stabilizing agent during the production of Ag-NPs. Pullulan-assisted synthesis under gamma irradiation was successfully developed to obtain Ag-NPs, which was characterized by UV-Vis, XRD, TEM, and Zeta potential analysis. Pullulan was used as a stabilizer and template for the growth of silver nanoparticles, while gamma radiation was modified to be selective to reduce silver ions. The formation of Ag-NPs was confirmed using UV-Vis spectra by showing a surface plasmon resonance (SPR) band in the region of 410-420 nm. As observed by TEM images, it can be said that by increasing the radiation dose, the particle size decreases, resulting in a mean diameter of Ag-NPs ranging from 40.97 to 3.98 nm. The XRD analysis confirmed that silver metal structures with a face-centered cubic (FCC) crystal were present, while TEM images showed a spherical shape with smooth edges. XRD also demonstrated that increasing the dose of gamma radiation increases the crystallinity at a high purity of Ag-NPs. As examined by zeta potential, the synthesized Ag-NP/PL was negatively charged with high stability. Ag-NP/PL was then analysed for antimicrobial activity against , and it was found that it had high antibacterial activity. It is found that the adoption of radiation doses results in a stable and green reduction process for silver nanoparticles.
即使在今天，生产具有独特性能的纯银纳米颗粒（Ag-NPs）仍然是一项挑战。在本研究中，采用辐射诱导法从天然普鲁兰多糖（PL）合成银纳米颗粒（Ag-NPs）。众所周知，普鲁兰多糖被视为一种微生物多糖，这使其适合在Ag-NPs生产过程中充当还原剂和稳定剂。成功开发了γ辐射下普鲁兰多糖辅助合成法以获得Ag-NPs，并通过紫外可见光谱、X射线衍射、透射电子显微镜和zeta电位分析对其进行了表征。普鲁兰多糖用作银纳米颗粒生长的稳定剂和模板，同时对γ辐射进行改性以选择性地还原银离子。通过紫外可见光谱在410-420nm区域显示表面等离子体共振（SPR）带，证实了Ag-NPs的形成。从透射电子显微镜图像观察可知，可以说随着辐射剂量的增加，粒径减小，导致Ag-NPs的平均直径在40.97至3.98nm范围内。X射线衍射分析证实存在面心立方（FCC）晶体结构的银金属，而透射电子显微镜图像显示边缘光滑的球形。X射线衍射还表明增加γ辐射剂量可提高Ag-NPs高纯度下的结晶度。通过zeta电位检测，合成的Ag-NP/PL带负电荷且具有高稳定性。然后分析了Ag-NP/PL对[未提及具体对象]的抗菌活性，发现其具有高抗菌活性。发现采用辐射剂量可实现银纳米颗粒稳定且绿色的还原过程。
