Mlynarcik Patrik, Dolejska Monika, Vagnerova Iva, Petrzelova Jana, Sukkar Iva, Zdarska Veronika, Kolar Milan
Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 77515 Olomouc, Czech Republic.
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic.
Life (Basel). 2021 Oct 13;11(10):1079. doi: 10.3390/life11101079.
Increasing antimicrobial resistance in nosocomial pathogens, such as , is becoming a serious threat to public health. It is necessary to detect -lactamase-producing microorganisms in clinical settings to be able to control the spread of carbapenem resistance. This study was conducted to evaluate the presence of -lactamases in a selected clinical isolate of of ST2/ST195 and to characterize possible enzymes, as well as its -lactam resistome, using PCR and whole-genome sequencing analysis. PCR and sequencing confirmed that the isolate harbored five gene alleles, namely, , , , and , as well as aminoglycosides, macrolides, sulfonamides and tetracyclines resistance determinants, which were either chromosomally and/or plasmid located. Furthermore, a gene order comparison using MAUVE alignment showed multiple changes compared with the clinical isolate of Malaysian AC30 genome and 76 regions with high homology. This study suggests that resistance to -lactams in this isolate is mainly due to an overproduction of -lactamases in combination with other resistance mechanism (efflux pump system).
医院病原体（如……）中日益增加的抗菌药物耐药性正成为对公众健康的严重威胁。有必要在临床环境中检测产β-内酰胺酶的微生物，以便能够控制碳青霉烯类耐药性的传播。本研究旨在评估ST2/ST195型某临床分离株中β-内酰胺酶的存在情况，并使用PCR和全基因组测序分析来鉴定可能的酶及其β-内酰胺耐药基因组。PCR和测序证实该分离株携带五个bla基因等位基因，即……以及氨基糖苷类、大环内酯类、磺胺类和四环素类耐药决定簇，这些基因决定簇位于染色体和/或质粒上。此外，使用MAUVE比对进行的基因顺序比较显示，与马来西亚某分离株AC30基因组的临床分离株相比有多处变化，且有76个区域具有高度同源性。本研究表明，该分离株对β-内酰胺类药物的耐药性主要是由于β-内酰胺酶的过量产生以及其他耐药机制（外排泵系统）共同作用。
