Department of Prosthodontics, Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, 310006, China.
Sci Rep. 2021 Oct 25;11(1):21022. doi: 10.1038/s41598-021-00497-y.
Peri-implantitis is a common reversible disease after tooth implantation, caused by a variety of pathogenic microorganisms. Based on non-surgical or surgical treatment principles, supplementation by local or systemic drugs might enhance treatment efficacy. Porphyromonas gingivalis (Pg) (ATCC 33,277) and Prevotella intermedius (Pi) (ATCC 25,611) were used as test strains. The effects of Pln 149 on the biofilm formation and growth of four periodontal pathogens were evaluated by RT-PCR, fluorescence microscopy, and scanning electron microscopy. The antibacterial mechanism was tested by the patch-clamp technique. The cytotoxicity of Pln 149 (125 µg/ml) to bone marrow stromal cell (BMSC) was assessed using an MTT assay. Pln 149 exhibited significant inhibitory effects on Pg and Pi (P < 0.05), with significant differences in the biofilm images of fluorescence microscope and scanning electron microscope (P < 0.05). Pln 149 could change the sodium channel currents and exerted no cytotoxicity on bone marrow stromal cell. Pln 149 could inhibit the biofilm formation and growth of periodontal pathogens. Considering the absence of antimicrobial resistance and cytotoxicity, we suggest that the Pln 149 from Lactobacillus plantarum 149 might be a promising option for managing peri-implantitis.
种植体周围炎是一种常见的种植体术后可逆性疾病，由多种致病微生物引起。基于非手术或手术治疗原则，局部或全身药物补充可能会提高治疗效果。使用牙龈卟啉单胞菌（Pg）（ATCC 33,277）和中间普氏菌（Pi）（ATCC 25,611）作为测试菌株。通过 RT-PCR、荧光显微镜和扫描电子显微镜评估 Pln 149 对四种牙周病病原体生物膜形成和生长的影响。通过膜片钳技术测试抗菌机制。使用 MTT 测定法评估 Pln 149（125μg/ml）对骨髓基质细胞（BMSC）的细胞毒性。Pln 149 对 Pg 和 Pi 表现出显著的抑制作用（P < 0.05），荧光显微镜和扫描电子显微镜的生物膜图像有显著差异（P < 0.05）。Pln 149 可以改变钠通道电流，对骨髓基质细胞没有细胞毒性。Pln 149 可以抑制牙周病病原体的生物膜形成和生长。鉴于不存在抗菌耐药性和细胞毒性，我们建议来自植物乳杆菌 149 的 Pln 149 可能是治疗种植体周围炎的有前途的选择。
