Zhao Feng, Deng Yingjun, Du Guanchao, Liu Shengjing, Guo Jun, Wang Hao, Chen Yazhou, Wang Fu, Geng Qiang
Department of Andrology, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No. 1 Xiyuan Playground, Zhongzhi Road, Beijing 100091, China.
Department of Andrology, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314 Anshan West Road, Tianjin 300193, China.
Evid Based Complement Alternat Med. 2021 Oct 16;2021:8281506. doi: 10.1155/2021/8281506. eCollection 2021.
The traditional Chinese medicines and are often combined to treat male infertility, but the specific therapeutic mechanism is not clear. Therefore, this study applies a network pharmacology approach to investigate the possible mechanism of action of the drug pair - (PAA) in the treatment of male infertility.
Relevant targets for PAA treatment of male infertility are obtained through databases. Protein-protein interactions (PPIs) are constructed through STRING database and screen core targets, and an enrichment analysis is conducted through the Metascape platform. Finally, molecular docking experiments were carried out to evaluate the affinity between the target protein and the ligand of PAA.
The active ingredients of 112 PAA, 980 corresponding targets, and 374 effective targets of PAA for the treatment of male infertility were obtained, which are related to PI3K-Akt signaling pathway, HIF-1 signaling pathway, AGE-RAGE signaling pathway, IL-17 signaling pathway, and thyroid hormone signaling pathway.
In this study, using a network pharmacology method, we preliminarily analyzed the effective components and action targets of the PAA. We also explored the possible mechanism of action of PAA in treating male infertility. They also lay a foundation for expanding the clinical application of PAA and provide new ideas and directions for further research on the mechanisms of action of the PAA and its components for male infertility treatment.
中药和常联合用于治疗男性不育症，但其具体治疗机制尚不清楚。因此，本研究应用网络药理学方法探讨药对-（PAA）治疗男性不育症的可能作用机制。
通过数据库获取PAA治疗男性不育症的相关靶点。通过STRING数据库构建蛋白质-蛋白质相互作用（PPI）并筛选核心靶点，通过Metascape平台进行富集分析。最后进行分子对接实验，评估靶点蛋白与PAA配体之间的亲和力。
获得了112种PAA的活性成分、980个相应靶点以及374个PAA治疗男性不育症的有效靶点，这些靶点与PI3K-Akt信号通路、HIF-1信号通路、AGE-RAGE信号通路、IL-17信号通路和甲状腺激素信号通路相关。
本研究采用网络药理学方法，初步分析了PAA的有效成分和作用靶点。我们还探讨了PAA治疗男性不育症的可能作用机制。它们也为扩大PAA的临床应用奠定了基础，并为进一步研究PAA及其成分治疗男性不育症的作用机制提供了新的思路和方向。
