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印度尼西亚三种蝙蝠中蝙蝠冠状病毒的分子检测。

Molecular detection of bat coronaviruses in three bat species in Indonesia.

机构信息

Indonesian Research Center for Veterinary Science, Ministry of Agriculture, Bogor 16124, Indonesia.

Research Centre for Biology, Indonesian Institute of Sciences (LIPI), Bogor 16911, Indonesia.

出版信息

J Vet Sci. 2021 Nov;22(6):e70. doi: 10.4142/jvs.2021.22.e70. Epub 2021 Aug 9.

Abstract

Bats are an important reservoir of several zoonotic diseases. However, the circulation of bat coronaviruses (BatCoV) in live animal markets in Indonesia has not been reported. Genetic characterization of BatCoV was performed by sequencing partial RdRp genes. Real-time polymerase chain reaction based on nucleocapsid protein (N) gene and Enzyme-linked immunosorbent assay against the N protein were conducted to detect the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA and antibody, respectively. We identified the presence of BatCoV on , , and . The results showed that the BatCoV included in this study are from an unclassified coronavirus group. Notably, SARS-CoV-2 viral RNA and antibodies were not detected in the sampled bats.

摘要

蝙蝠是几种人畜共患病的重要宿主。然而,印度尼西亚活体动物市场中蝙蝠冠状病毒(BatCoV)的循环情况尚未有报道。通过测序部分 RdRp 基因对 BatCoV 进行遗传特征分析。基于核衣壳蛋白(N)基因的实时聚合酶链反应和针对 N 蛋白的酶联免疫吸附试验分别用于检测严重急性呼吸综合征冠状病毒 2(SARS-CoV-2)病毒 RNA 和抗体的存在。我们在、、和中鉴定出了 BatCoV 的存在。结果表明,本研究中包含的 BatCoV 属于未分类的冠状病毒群。值得注意的是,在采样蝙蝠中未检测到 SARS-CoV-2 病毒 RNA 和抗体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5335/8636659/219deb46440e/jvs-22-e70-g001.jpg

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