Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Double-First Class Discipline of Human-Animal Medicine, Jilin University, Changchun 130015, China.
Norman Bethune Health Science Center, Jilin University, Changchun 130015, China.
Curr Issues Mol Biol. 2021 Oct 11;43(3):1529-1547. doi: 10.3390/cimb43030108.
Melanocortin 1 receptor () is thought to be a marker of poor prognosis and a potential target for the treatment of melanoma. Studies have found that promotes several tumor behaviors, including cell proliferation and differentiation, pigment formation, and genome damage repair. Some single-nucleotide polymorphisms (SNPs) of are involved in the occurrence and development of melanoma. A few studies have reported a relationship between and colorectal cancer (CRC). In this research, our objective was to examine expression and SNPs and investigate their correlation with the clinicopathological features of human CRC tissues. We evaluated mRNA expression by performing bioinformatic analyses on human CRC expression datasets. We used Western blotting and RT-qPCR to compare expression in CRC tissues with that in normal tissues, and SNPs in CRC tissues were detected by PCR-direct sequencing (DS). The expression of was significantly decreased in CRC tissues compared with normal tissue, and its expression was negatively associated with expression, expression, and expression, and high expression was significantly associated with microsatellite instability (MSI). SNPs were also associated with the clinicopathological characteristics of CRC; for example, the rs2228479 locus genotype was correlated with status, and the rs885479 locus genotype was correlated with age and T stage. In conclusion, plays a crucial role in the progression of CRC and may be a marker of poor prognosis in CRC.
黑素皮质素 1 受体（）被认为是预后不良的标志物，也是治疗黑色素瘤的潜在靶点。研究发现，可促进多种肿瘤行为，包括细胞增殖和分化、色素形成以及基因组损伤修复。的一些单核苷酸多态性（SNPs）与黑色素瘤的发生和发展有关。一些研究报告了与结直肠癌（CRC）之间的关系。在这项研究中，我们的目的是研究表达和 SNPs，并探讨其与人类 CRC 组织临床病理特征的相关性。我们通过对人类 CRC 表达数据集进行生物信息学分析来评估 mRNA 的表达。我们使用 Western blot 和 RT-qPCR 比较 CRC 组织和正常组织中的表达，并通过 PCR 直接测序（DS）检测 CRC 组织中的 SNPs。与正常组织相比，CRC 组织中表达显著降低，其表达与表达、表达和表达呈负相关，高表达与微卫星不稳定性（MSI）显著相关。SNP 也与 CRC 的临床病理特征相关；例如，rs2228479 位点基因型与状态相关，rs885479 位点基因型与年龄和 T 分期相关。总之，在 CRC 的进展中发挥着重要作用，可能是 CRC 预后不良的标志物。
