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通过 ceRNA 网络构建研究 LH 对猪原始生殖细胞样细胞（pPGCLCs）的增殖作用。

The proliferation role of LH on porcine primordial germ cell-like cells (pPGCLCs) through ceRNA network construction.

机构信息

College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao, China.

Animal Husbandry General Station of Shandong Province, Jinan, China.

出版信息

Clin Transl Med. 2021 Oct;11(10):e560. doi: 10.1002/ctm2.560.

DOI:10.1002/ctm2.560
PMID:34709759
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8516341/
Abstract

BACKGROUND

The transdifferentiation of skin-derived stem cells (SDSCs) into primordial germ cell-like cells (PGCLCs) is one of the major breakthroughs in the field of stem cells research in recent years. This technology provides a new theoretical basis for the treatment of human infertility. However, the transdifferentiation efficiency of SDSCs to PGCLCs is very low, and scientists are still exploring ways to improve this efficiency or promote the proliferation of PGCLCs. This study aims to investigate the molecular mechanism of luteinising hormone (LH) to enhance porcine PGCLCs (pPGCLCs) proliferation.

RESULTS

In this study, we dissected the proliferation regulatory network of pPGCLCs by whole transcriptome sequencing, and the results showed that the pituitary-secreted reproductive hormone LH significantly promoted the proliferation of pPGCLCs. We combined whole transcriptome sequencing and related validation experiments to explore the mechanism of LH on the proliferation of pPGCLCs, and found that LH could affect the expression of Hippo signalling pathway-related mRNAs, miRNAs and lncRNAs in pPGCLCs.

CONCLUSIONS

For the first time, we found that LH promotes pPGCLCs proliferation through the competing endogenous RNA (ceRNA) regulatory networks and Hippo signalling pathway. This finding may help to elucidate the molecular mechanism by which LH promotes pPGCLCs proliferation.

摘要

背景

皮肤源性干细胞（SDSCs）向原始生殖细胞样细胞（PGCLCs）的转分化是近年来干细胞研究领域的重大突破之一。这项技术为治疗人类不孕提供了新的理论基础。然而，SDSCs 向 PGCLCs 的转分化效率非常低，科学家们仍在探索提高这种效率或促进 PGCLCs 增殖的方法。本研究旨在探讨促黄体生成素（LH）增强猪 PGCLCs（pPGCLCs）增殖的分子机制。

结果

本研究通过全转录组测序剖析了 pPGCLCs 的增殖调控网络，结果表明，垂体分泌的生殖激素 LH 显著促进了 pPGCLCs 的增殖。我们结合全转录组测序和相关验证实验，探讨了 LH 对 pPGCLCs 增殖的作用机制，发现 LH 能够影响 pPGCLCs 中 Hippo 信号通路相关 mRNA、miRNA 和 lncRNA 的表达。

结论

本研究首次发现，LH 通过竞争性内源 RNA（ceRNA）调控网络和 Hippo 信号通路促进 pPGCLCs 的增殖。这一发现可能有助于阐明 LH 促进 pPGCLCs 增殖的分子机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/f8a7ff2c8a09/CTM2-11-e560-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/0a48d0c26799/CTM2-11-e560-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/0cc1c3b4f0ba/CTM2-11-e560-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/60133768dfff/CTM2-11-e560-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/dc2d656a87f2/CTM2-11-e560-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/08b0cbddc91f/CTM2-11-e560-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/f8a7ff2c8a09/CTM2-11-e560-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/0a48d0c26799/CTM2-11-e560-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/0cc1c3b4f0ba/CTM2-11-e560-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/60133768dfff/CTM2-11-e560-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/dc2d656a87f2/CTM2-11-e560-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/08b0cbddc91f/CTM2-11-e560-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a2b/8516341/f8a7ff2c8a09/CTM2-11-e560-g005.jpg

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