Liao Yingnan, Zhu Liyuan, Wang Yan
Xiamen Key Laboratory of Cardiovascular Disease, Xiamen Cardiovascular Hospital, Xiamen University, Xiamen, China.
Int J Stem Cells. 2021 Nov 30;14(4):366-385. doi: 10.15283/ijsc21077.
With the in-depth study of heart development, many human cardiomyocytes (CMs) have been generated in a laboratory environment. CMs derived from pluripotent stem cells (PSCs) have been widely used for a series of applications such as laboratory studies, drug toxicology screening, cardiac disease models, and as an unlimited resource for cell-based cardiac regeneration therapy. However, the low maturity of the induced CMs significantly impedes their applicability. Scientists have been committed to improving the maturation of CMs to achieve the purpose of heart regeneration in the past decades. In this review, we take CMs maturation as the main object of discussion, describe the characteristics of CMs maturation, summarize the key regulatory mechanism of regulating maturation and address the approaches to promote CMs maturation. The maturation of CM is gradually improving due to the incorporation of advanced technologies and is expected to continue.
随着对心脏发育的深入研究，许多人类心肌细胞（CMs）已在实验室环境中生成。源自多能干细胞（PSCs）的CMs已被广泛应用于一系列领域，如实验室研究、药物毒理学筛选、心脏病模型，以及作为基于细胞的心脏再生治疗的无限资源。然而，诱导生成的CMs成熟度较低，这严重阻碍了它们的适用性。在过去几十年里，科学家们一直致力于提高CMs的成熟度，以实现心脏再生的目的。在这篇综述中，我们以CMs成熟为主要讨论对象，描述CMs成熟的特征，总结调节成熟的关键调控机制，并探讨促进CMs成熟的方法。由于先进技术的融入，CMs的成熟度正在逐步提高，并且有望持续下去。
