Ramsing Charis K, Gramaje David, Mocholí Sara, Agustí Javier, Cabello Sáenz de Santa María Félix, Armengol Josep, Berbegal Mónica
Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Valencia, Spain.
Instituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas, Universidad de la Rioja-Gobierno de La Rioja, Logroño, Spain.
Front Plant Sci. 2021 Oct 12;12:726461. doi: 10.3389/fpls.2021.726461. eCollection 2021.
Fungal grapevine trunk diseases (GTDs) are some of the most pressing threats to grape production worldwide. While these diseases are associated with several fungal pathogens, and are important contributors to esca and Petri diseases. Recent research has linked grapevine xylem diameter with tolerance to in commercial rootstocks. In this study, we screen over 25 rootstocks for xylem characteristics and tolerance to both and . Tolerance was measured by fungal incidence and DNA concentration (quantified via qPCR), while histological analyses were used to measure xylem characteristics, including xylem vessels diameter, density, and the proportion of the stem surface area covered by xylem vessels. Rootstocks were grouped into different classes based on xylem characteristics to assess the potential association between vasculature traits and pathogen tolerance. Our results revealed significant differences in all the analyzed xylem traits, and also in DNA concentration for both pathogens among the tested rootstocks. They corroborate the link between xylem vessels diameter and tolerance to . In , the rootstocks with the widest xylem diameter proved the most susceptible. This relationship between vasculature development and pathogen tolerance has the potential to inform both cultivar choice and future rootstock breeding to reduce the detrimental impact of GTDs worldwide.
葡萄树干真菌病害（GTDs）是全球葡萄生产面临的最紧迫威胁之一。虽然这些病害与多种真菌病原体有关，并且是葡萄枝干病害和佩特里氏病的重要成因。最近的研究表明，商业砧木中葡萄木质部直径与对[病害名称未明确]的耐受性有关。在本研究中，我们筛选了超过25种砧木，以研究其木质部特征以及对[病害名称未明确]和[病害名称未明确]的耐受性。通过真菌发病率和DNA浓度（通过qPCR定量）来测定耐受性，同时利用组织学分析来测量木质部特征，包括木质部导管直径、密度以及木质部导管覆盖的茎表面积比例。根据木质部特征将砧木分为不同类别，以评估维管系统特征与病原体耐受性之间的潜在关联。我们的结果显示，在所有分析的木质部性状以及测试砧木中两种病原体的DNA浓度方面均存在显著差异。这些结果证实了木质部导管直径与对[病害名称未明确]的耐受性之间的联系。在[病害名称未明确]方面，木质部直径最宽的砧木被证明最易感病。维管系统发育与病原体耐受性之间的这种关系有可能为品种选择和未来砧木育种提供参考，以减少全球范围内GTDs的有害影响。
