Ma Nan, Yin Dengpan, Liu Ying, Gao Ziyong, Cao Yu, Chen Tongtong, Huang Ziyi, Jia Qiaojun, Wang Dekai
Key Laboratory of Plant Secondary Metabolism Regulation in Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China.
Front Microbiol. 2023 Aug 1;14:1220431. doi: 10.3389/fmicb.2023.1220431. eCollection 2023.
Golden buckwheat (, also known as ) is a traditional edible herbal medicinal plant with a large number of secondary metabolites and is considered to be a source of therapeutic compounds. Different ecological environments have a significant impact on their compound content and medicinal effects. However, little is known about the interactions between soil physicochemical properties, the rhizosphere, endophytic fungal communities, and secondary metabolites in . In this study, the rhizosphere soil and endophytic fungal communities of in five different ecological regions in China were identified based on high-throughput sequencing methods. The correlations between soil physicochemical properties, active components (total saponins, total flavonoids, proanthocyanidin, and epicatechin), and endophytic and rhizosphere soil fungi of were analyzed. The results showed that soil pH, soil N, OM, and P were significantly correlated with the active components of . Among them, epicatechin, proanthocyanidin, and total saponins were significantly positively correlated with soil pH, while proanthocyanidin content was significantly positively correlated with STN, SAN, and OM in soil, and total flavone content was significantly positively correlated with P in soil. In soil microbes, , Ascomycota_unclassified, , Fungi_unclassified, Agaricomycetes_unclassified, , and were significantly related to key secondary metabolites of . and Meripilaceae_unclassified were significantly related to key secondary metabolites in the rhizome. This study presents a new opportunity to deeply understand soil-plant-fungal symbioses and secondary metabolites in , as well as provides a scientific basis for using biological fertilization strategies to improve the quality of .
金荞麦（，也被称为 ）是一种传统的可食用草本药用植物，含有大量次生代谢产物，被认为是治疗化合物的来源。不同的生态环境对其化合物含量和药用效果有显著影响。然而，关于金荞麦土壤理化性质、根际、内生真菌群落和次生代谢产物之间的相互作用知之甚少。在本研究中，基于高通量测序方法鉴定了中国五个不同生态区域内金荞麦的根际土壤和内生真菌群落。分析了金荞麦土壤理化性质、活性成分（总皂苷、总黄酮、原花青素和表儿茶素）与内生真菌和根际土壤真菌之间的相关性。结果表明，土壤pH值、土壤氮、有机质和磷与金荞麦的活性成分显著相关。其中，表儿茶素、原花青素和总皂苷与土壤pH值显著正相关，原花青素含量与土壤中的全氮、有效氮和有机质显著正相关，总黄酮含量与土壤中的磷显著正相关。在土壤微生物中，、未分类的子囊菌门、、未分类的真菌、未分类的伞菌纲、和与金荞麦的关键次生代谢产物显著相关。和未分类的多孔菌科与根茎中的关键次生代谢产物显著相关。本研究为深入了解金荞麦土壤 - 植物 - 真菌共生关系和次生代谢产物提供了新机会，也为利用生物施肥策略提高金荞麦品质提供了科学依据。
