Department of Ophthalmology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi, 329-0431, Japan.
Department of Ophthalmology Japan Community Healthcare Organization, Tokyo Shinjuku Medical Center, Shinjuku-ku, Tokyo, Japan.
Graefes Arch Clin Exp Ophthalmol. 2022 Mar;260(3):747-758. doi: 10.1007/s00417-021-05445-0. Epub 2021 Oct 29.
To classify macular neovascularization (MNV) based on pachychoroid and drusen features and to examine the aqueous humor cytokine signatures of each group.
In total, 106 consecutive eyes with treatment-naïve MNV and 104 control eyes were examined. The aqueous humor concentrations of 15 cytokines were compared among the MNV groups classified based on the presence of drusen and/or pachychoroid features. Multidimensional scaling analysis was used to visualize the similarity level of the MNV subtypes according to their cytokine profiles.
Thirty-one, 18, 43, and 10 eyes were classified into the pachychoroid-associated, drusen-associated, pachychoroid/drusen-associated, and non-drusen/non-pachychoroid MNV groups, respectively. Compared with the control group, cytokines were differently upregulated among the MNV groups. CRP and CXCL12 were significantly upregulated in all MNV groups, whereas CXCL13 and IL-8 were significantly upregulated in three MNV groups, excluding the non-pachychoroid/non-drusen-associated MNV group. Ang-2 was significantly upregulated in three MNV groups except the drusen-associated MNV group. PlGF was significantly upregulated in the pachychoroid-associated and drusen-associated MNV groups. CCL-2 was significantly upregulated in the pachychoroid-associated and pachychoroid/drusen-associated MNV groups. VEGF was downregulated in the pachychoroid-associated and drusen-associated MNV groups, respectively. Multidimensional scaling analysis showed a distinct cytokine profile for each MNV group.
All MNV groups showed distinct cytokine profiles. Eyes with "neovascular age-related macular degeneration with drusen and concomitant pachychoroid" may share a similar etiology to those with "pachychoroid neovasculopathy" and "choroidal neovascularization with drusen," but have a distinct etiology to those without these. These findings suggest the importance of evaluating drusen and the choroid during the diagnosis of neovascular age-related macular degeneration.
基于脉络膜增厚和玻璃膜疣特征对黄斑新生血管（MNV）进行分类，并研究各组房水细胞因子特征。
共纳入 106 例未经治疗的 MNV 患眼和 104 例对照眼。比较基于玻璃膜疣和/或脉络膜增厚特征分类的 MNV 组之间房水中 15 种细胞因子的浓度。多维尺度分析用于根据 MNV 亚型的细胞因子谱来可视化其相似性水平。
31、18、43 和 10 只眼分别被归类为脉络膜增厚相关、玻璃膜疣相关、脉络膜增厚/玻璃膜疣相关和非玻璃膜疣/非脉络膜增厚相关 MNV 组。与对照组相比，MNV 各组中的细胞因子水平存在差异上调。CRP 和 CXCL12 在所有 MNV 组中均显著上调，而 CXCL13 和 IL-8 在除非玻璃膜疣/非脉络膜增厚相关 MNV 组之外的三组 MNV 中显著上调。Ang-2 在除玻璃膜疣相关 MNV 组之外的三组 MNV 中显著上调。PlGF 在脉络膜增厚相关和玻璃膜疣相关 MNV 组中显著上调。CCL-2 在脉络膜增厚相关和脉络膜增厚/玻璃膜疣相关 MNV 组中显著上调。VEGF 在脉络膜增厚相关和玻璃膜疣相关 MNV 组中分别下调。多维尺度分析显示每个 MNV 组都有独特的细胞因子谱。
所有 MNV 组均显示出独特的细胞因子谱。具有“玻璃膜疣伴脉络膜增厚的新生血管性年龄相关性黄斑变性”的眼可能与“脉络膜新生血管病”和“伴玻璃膜疣的脉络膜新生血管”具有相似的病因，但与不具有这些特征的眼有不同的病因。这些发现提示在诊断新生血管性年龄相关性黄斑变性时评估玻璃膜疣和脉络膜的重要性。
