School of Life Sciences, B.S.Abdur Rahman Crescent Institute of Science and Technology, Chennai, 600048, India.
Centre of MicroAlgal and Nano Biotechnology (CeMANT), Crescent Innovation and Incubation Council (CIIC), B.S.Abdur Rahman Crescent Institute of Science and Technology, Chennai, 600048, India.
Appl Biochem Biotechnol. 2022 Mar;194(3):1390-1400. doi: 10.1007/s12010-021-03728-0. Epub 2021 Oct 29.
Generally, rapid detection of viral infection is necessary for preventing the virus from spreading among people in a society as a pandemic. Although there are many effective standard techniques used for virus identification, they are laborious, required skilled person to handle and time-consuming. Particularly, the detection of viral infection involved in the isolation and nucleic acid detection by collecting specimens (sample) from the appropriate sites. For instance, oral or nasal swab, nasopharyngeal or tracheal extract, lung tissue, blood, sputum and feces are collected in order to investigate the pandemic, COVID-19 for the effective and rapid diagnosis and eventually for the treatment. In this mini-review, it is summarized that the advanced testing methods which include RNA, immunologic and radiological based tests that could be used to detect COVID-19 and their cost, reliability and functionality are discussed in this review. This mini-review might help the researcher and health care sector to plan the diagnostic procedures as per the severity of the new infection, COVID-19.
通常,快速检测病毒感染对于防止病毒在社会中传播以形成大流行是必要的。尽管有许多用于病毒鉴定的有效标准技术,但它们既繁琐,又需要熟练的人员来操作,而且耗时。特别是,病毒感染的检测涉及到通过从适当部位采集标本(样本)来进行的分离和核酸检测。例如,为了调查大流行,需要采集口腔或鼻腔拭子、鼻咽或气管提取物、肺组织、血液、痰液和粪便,以便进行有效的快速诊断,并最终进行治疗。在这篇小型综述中,总结了包括基于 RNA、免疫和影像学的检测方法,可用于检测 COVID-19 及其成本、可靠性和功能。本综述可能有助于研究人员和医疗保健部门根据新感染 COVID-19 的严重程度来规划诊断程序。