Life Sciences Institute, Zunyi Medical University, Zunyi 563006, PR China.
Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics (Capital Medical University), Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, PR China.
Syst Appl Microbiol. 2021 Nov;44(6):126275. doi: 10.1016/j.syapm.2021.126275. Epub 2021 Oct 18.
Two novel strains KQZ13P-1 and MAQZ13P-2 were isolated from bark of Sonneratia apetala collected from Maowei sea Mangrove Nature Reserve in Guangxi Zhuang Autonomous Region, China. Two strains were Gram-positive, aerobic, non-spore-forming, no diffusion pigment actinobacterial strains and investigated by a polyphasic approach to determine their taxonomic position. The average nucleotide identity (ANI) value and the digital DNA-DNA hybridization (dDDH) value between the two strains were 99.9% and 99.7%, respectively, suggesting that they belonged to the same species. The ANI and dDDH values between strain KQZ13P-1 and five Phycicoccus species were 74.4-95.3% and 20.1-61.5%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that the two strains were member of the genus Phycicoccus and were closely related to P. jejuensis NRRL B-24460 (99.2% sequence similarity), followed by P. ginsengisoli DCY87 (97.5-97.6%). Moreover, based on 88 core genes, the phylogenomic tree indicated that the two strains clustered with P. jejuensis NRRL B-24460. The cell-wall peptidoglycan of both strains contained meso-diaminopimelic acid. The major fatty acids in two strains were Cω8c, iso-C and iso-C. The major polar lipids included diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylinositol (PI). Based on phylogenetic, phenotypic and chemotaxonomic analysis, strains KQZ13P-1 and MAQZ13P-2 represent a novel species of the genus Phycicoccus, for which the name Phycicoccus mangrovi sp. nov. is proposed. The type strain is KQZ13P-1 (=CGMCC 1.18973 = JCM 34556).
两株新的分离株 KQZ13P-1 和 MAQZ13P-2 从广西壮族自治区茅尾海红树林自然保护区采集的海桑树皮中分离得到。这两株菌均为革兰氏阳性、需氧、不产芽孢的放线菌，通过多相分类方法研究它们的分类地位。两株菌的平均核苷酸同源性（ANI）值和数字 DNA-DNA 杂交（dDDH）值分别为 99.9%和 99.7%，表明它们属于同一物种。KQZ13P-1 菌株与五个 Phycicoccus 种的 ANI 和 dDDH 值分别为 74.4-95.3%和 20.1-61.5%。基于 16S rRNA 基因序列的系统发育分析表明，这两株菌均属于 Phycicoccus 属，与 Phycicoccus jejuensis NRRL B-24460 （99.2%序列相似性）最为密切，其次是 P. ginsengisoli DCY87（97.5-97.6%）。此外，基于 88 个核心基因，系统基因组树表明，这两株菌与 Phycicoccus jejuensis NRRL B-24460 聚类。两株菌的细胞壁肽聚糖均含有 meso-二氨基庚二酸。两株菌的主要脂肪酸为 Cω8c、iso-C 和 iso-C。主要极性脂包括双磷脂酰甘油（DPG）、磷脂酰乙醇胺（PE）和磷脂酰肌醇（PI）。基于系统发育、表型和化学分类分析，菌株 KQZ13P-1 和 MAQZ13P-2 代表 Phycicoccus 属的一个新种，命名为 Phycicoccus mangrovi sp. nov.。模式菌株为 KQZ13P-1（=CGMCC 1.18973=JCM 34556）。
