Life Sciences Institute, Zunyi Medical University, Zunyi 563006, PR China.
Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005310.
A new endophytic bacterium, designated strain MQZ13P-4 was isolated from collected from Maowei sea Mangrove Nature Reserve in Guangxi Zhuang Autonomous Region, PR China. The 16S rRNA gene sequence similarity between strain MQZ13P-4 and its closest phylogenetic neighbour CBS5Q-3 was 97.9 %. Phylogenetic analyses using 16S rRNA gene sequences and whole-genome sequences showed that strain MQZ13P-4 formed a distinct lineage with CBS5Q-3, 40Bstr34 and JCM 30119. The draft genome of strain MQZ13P-4 was 5 153 243 bp in size and its DNA G+C content was 68.1 mol%. Comparative genome analysis revealed that the average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values among strain MQZ13P-4 and other related species were below the cut-off levels of 95, 70 and 95.5 %, respectively. The cell-wall peptidoglycan of strain MQZ13P-4 contained -diaminopimelic acid as the diagnostic diamino acid. The respiratory quinone was Q-10. The major cellular fatty acid was C 7. The polar lipids comprised phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminolipids and two unidentified lipids. Strain MQZ13P-4 had a typical chemical compositions of fatty acids, lipids, quinones and diagnostic diamino acid for species, but could be distinguished from known species of the genus . Based on polyphasic evidence, strain MQZ13P-4 represents novel species of the genus , for which the name sp. nov. is proposed. The type strain is MQZ13P-4 (=CGMCC 1.18727=JCM 34333).
一株新的内生细菌，命名为菌株 MQZ13P-4，从中国广西壮族自治区茅尾海红树林自然保护区采集的植物中分离得到。菌株 MQZ13P-4 与最接近的系统发育近邻 CBS5Q-3 的 16S rRNA 基因序列相似性为 97.9%。基于 16S rRNA 基因序列和全基因组序列的系统发育分析表明，菌株 MQZ13P-4 与 CBS5Q-3、40Bstr34 和 JCM 30119 形成了一个独特的分支。菌株 MQZ13P-4 的基因组草图大小为 5153243bp，其 DNA G+C 含量为 68.1mol%。比较基因组分析表明，菌株 MQZ13P-4 与其他相关种之间的平均核苷酸同一性、数字 DNA-DNA 杂交和平均氨基酸同一性值均低于 95%、70%和 95.5%的截止值。菌株 MQZ13P-4 的细胞壁肽聚糖含有 -二氨基庚二酸作为诊断二氨基酸。呼吸醌为 Q-10。主要细胞脂肪酸为 C7。极性脂包括磷脂酰胆碱、磷脂酰乙醇胺、磷脂单甲醚乙醇胺、磷脂酰甘油、双磷脂酰甘油、两种未鉴定的胺脂和两种未鉴定的脂类。菌株 MQZ13P-4 的脂肪酸、脂类、醌类和诊断二氨基酸的化学组成具有典型的种特征，但与该属的已知种可区分。基于多相证据，菌株 MQZ13P-4 代表了该属的一个新种，提议将其命名为 sp. nov.。模式菌株为 MQZ13P-4（=CGMCC 1.18727=JCM 34333）。
