Ciurea Cristina Nicoleta, Santini Ario, Mare Anca Delia, Kosovski Irina Bianca, Toma Felicia, Vintila Camelia, Pintea-Simon Ionela Anca, Man Adrian
Department of Microbiology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Targu Mures Romania.
Doctoral school, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Targu Mures Romania.
J Crit Care Med (Targu Mures). 2021 Aug 5;7(3):217-226. doi: 10.2478/jccm-2021-0016. eCollection 2021 Jul.
Lower respiratory tract secretions (LRTS) like sputum and tracheal aspirates are frequently sent to the microbiology laboratory from patients with various respiratory pathologies. Improper collection techniques can lead to false-positive results, resulting in improper therapy.
To determine the percentage of contaminated samples sent to the microbiology laboratory, to establish the prevalence of spp. in non-contaminated samples and therefore, the presence of spp. originating in lower respiratory tract infections. Material and.
A 10-year data survey was conducted to assess the differences in prevalence from contaminated versus non-contaminated samples, assessed and categorised by Bartlett grading system, and to emphasise the importance of quality control for potentially contaminated samples. The data were analysed according to gender, age, referring departments, and the species of . For the statistical analysis, Kruskal-Wallis and Fisher tests were used, and the alpha value was set for 0.5.
The prevalence of spp. in all analysed samples was 31.60%. After excluding the contaminated samples, the actual prevalence was 27.66%. Of all sputum samples, 31.6% were contaminated. Patients aged more than 40 years old were more prone to provide contaminated sputum samples. is more prevalent in non-contaminated sputum samples. In both sputum and tracheal aspirates, the chances of identifying a single species are higher than the chances of identifying multiple species.
The study emphasises the importance of assessing the quality of sputum samples because of the high number of improperly collected samples sent to the microbiology laboratory.
痰液和气管吸出物等下呼吸道分泌物（LRTS）经常从患有各种呼吸道疾病的患者处送往微生物实验室。采集技术不当可能导致假阳性结果，从而导致治疗不当。
确定送往微生物实验室的受污染样本的百分比，确定非受污染样本中 spp. 的流行情况，进而确定源自下呼吸道感染的 spp. 的存在情况。材料与方法：进行了一项为期10年的数据调查，以评估受污染样本与非受污染样本在 流行率上的差异，通过巴特利特分级系统进行评估和分类，并强调对潜在受污染样本进行质量控制的重要性。根据性别、年龄、送检科室和 的种类对数据进行分析。对于统计分析，使用了克鲁斯卡尔 - 沃利斯检验和费舍尔检验，设定的α值为0.5。
所有分析样本中 spp. 的流行率为31.60%。排除受污染样本后，实际流行率为27.66%。在所有痰液样本中，31.6% 受到污染。40岁以上的患者更容易提供受污染的痰液样本。 在未受污染的痰液样本中更为普遍。在痰液和气管吸出物中，鉴定出单一菌种的可能性高于鉴定出多种菌种的可能性。
该研究强调了评估痰液样本质量的重要性，因为送往微生物实验室的采集不当的样本数量众多。
