El-Safty Samy M, El-Wakiel Nadia, El-Oleimy Gehan, Gaber Mohamed, El-Sayed Yusif S
Department of Dental Biomaterials, Faculty of Dentistry, Tanta University, Tanta 31527, Egypt.
Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt.
Int J Biomater. 2021 Oct 23;2021:1925388. doi: 10.1155/2021/1925388. eCollection 2021.
To synthesize and characterize a novel Al complex with 2-(2-hydroxyphenyl)-1H-benzimidazole (HL) to be added to a restorative glass ionomer cement (GIC) to enhance its antimicrobial activities and to evaluate the Vickers microhardness (HV) and fluoride release (FR) of the modified GIC.
Al complex was synthesized by the addition of 1 mmol (0.210 g) of HL to 1 mmol (0.342 g) of aluminum sulfate in ethanol. The resulting solution was then refluxed under stirring for 24 h and then collected by filtration and dried in a vacuum desiccator over an anhydrous CaCl. Characterization of Al complex was carried out by Fourier transform infrared spectroscopy (FTIR), elemental microanalysis, thermal gravimetric analysis (TGA), molar conductance, H NMR spectra, and electron impact-mass spectrometry. The antimicrobial activity of Al complex-modified GIC (Al-GIC) was studied by the "cut plug method" against Gram-negative bacteria () and Gram-positive bacteria (, , and ) and fungi (). HV was evaluated by a digital microhardness tester (Zwick/Roell, Indentec, ZHV-S, West Midlands, England). Fluoride levels in ppm were obtained using the ion-selective electrode connected to a digital meter. A one-way ANOVA and Bonferroni test were used to analyze the data with the significance level established at ≤ 0.05.
Synthesis of Al complex was confirmed by FTIR, elemental microanalysis TGA, molar conductance, H NMR spectra, and electron impact-mass spectrometry. Al-GICs exhibited an enhanced antibacterial activity against all studied microorganisms as confirmed by the growth of inhibition zones compared to control GIC (C-GIC). Though there was a slight reduction in HV and FR with increasing the added percent of Al complex, no significant differences were found between the studied groups.
Addition of Al complex to GIC powder enhanced the antimicrobial activity of GIC materials. As there was a negligible insignificant reduction in HV and FR upon the addition of Al complex, Al-GICs can be used with a guaranteed degree of clinical success.
合成并表征一种与2-(2-羟基苯基)-1H-苯并咪唑(HL)形成的新型铝配合物，将其添加到修复性玻璃离子水门汀(GIC)中以增强其抗菌活性，并评估改性GIC的维氏显微硬度(HV)和氟释放(FR)。
在乙醇中，将1 mmol(0.210 g)的HL加入到1 mmol(0.342 g)的硫酸铝中合成铝配合物。然后将所得溶液在搅拌下回流24小时，然后过滤收集并在无水氯化钙上的真空干燥器中干燥。通过傅里叶变换红外光谱(FTIR)、元素微量分析、热重分析(TGA)、摩尔电导率、1H NMR光谱和电子轰击质谱对铝配合物进行表征。采用“切塞法”研究了铝配合物改性GIC(Al-GIC)对革兰氏阴性菌( )和革兰氏阳性菌( 、 、 )及真菌( )的抗菌活性。通过数字显微硬度测试仪(Zwick/Roell，Indentec，ZHV-S，英格兰西米德兰兹)评估HV。使用连接到数字仪表的离子选择性电极获得以ppm为单位的氟含量。采用单因素方差分析和Bonferroni检验分析数据，显著性水平设定为 ≤ 0.05。
通过FTIR、元素微量分析、TGA、摩尔电导率、1H NMR光谱和电子轰击质谱证实了铝配合物的合成。与对照GIC(C-GIC)相比，Al-GIC对所有研究的微生物均表现出增强的抗菌活性，抑菌圈的生长证实了这一点。尽管随着铝配合物添加百分比的增加，HV和FR略有降低，但研究组之间未发现显著差异。
向GIC粉末中添加铝配合物可增强GIC材料的抗菌活性。由于添加铝配合物后HV和FR的降低可忽略不计，Al-GIC可在保证一定临床成功率的情况下使用。
