红细胞输注诱导的非转铁蛋白结合铁促进人血清中铜绿假单胞菌生物膜的形成和导管化小鼠的死亡率。
Red blood cell transfusion-induced non-transferrin-bound iron promotes Pseudomonas aeruginosa biofilms in human sera and mortality in catheterized mice.
机构信息
Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, NY, USA.
Center for Comparative Medicine, Massachusetts General Hospital, Boston, MA, USA.
出版信息
Br J Haematol. 2022 Feb;196(4):1105-1110. doi: 10.1111/bjh.17934. Epub 2021 Nov 2.
Abstract
Transfusion of storage-damaged red blood cells (RBCs) increases non-transferrin-bound iron (NTBI) levels in humans. This can potentially enhance virulence of microorganisms. In this study, Pseudomonas aeruginosa replication and biofilm production in vitro correlated with NTBI levels of transfused subjects (R = 0·80; P < 0·0001). Transfusion of stored RBCs into catheterized mice enhanced P. aeruginosa virulence and mortality in vivo, while pre-administration of apotransferrin reduced NTBI levels improving survival (69% vs 27% mortality; P < 0·05). These results suggest that longer RBC storage, by modulating the bioavailability of iron, may increase the risk of P. aeruginosa biofilm-related infections in transfused patients.
摘要
储存损伤的红细胞（RBC）的输血会增加人体内非转铁蛋白结合铁（NTBI）的水平。这可能会增强微生物的毒力。在这项研究中，铜绿假单胞菌在体外的复制和生物膜生成与输血患者的 NTBI 水平相关（R = 0·80；P < 0·0001）。将储存的 RBC 输注到置管的小鼠体内会增强铜绿假单胞菌的毒力并导致其在体内死亡，而预先给予脱铁转铁蛋白会降低 NTBI 水平从而提高存活率（死亡率为 69% vs 27%；P < 0·05）。这些结果表明，通过调节铁的生物利用度，RBC 的储存时间延长可能会增加输血患者与铜绿假单胞菌生物膜相关感染的风险。
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