Department of Therapeutic Chemistry, National Research Centre, Giza, Egypt.
Chemistry of Natural Compounds Department, National Research Centre, Giza, Egypt.
Biomarkers. 2022 Feb;27(1):35-43. doi: 10.1080/1354750X.2021.2002412. Epub 2021 Nov 17.
Carbon tetrachloride (CCl) induces oxidative stress in various tissues by altering antioxidants defense system. Recently, there has been a substantial use of phytotherapy to treat different diseases.
This study was designed to evaluate the curative effect of (Forssk.) Benth et Hook (Family Asteraceae) aerial parts ethanol extract against CCl induced toxicity in rats kidneys.
Nephrotoxicity was induced by intraperitoneal injection with CCl in a dose of 0.5 mL/kg b.wt./twice a week for six consecutive weeks. Serum kidney function tests, oxidative stress markers, inflammatory cytokines, nephrotoxicity biomarkers and histopathological observation were evaluated.
CCl increased serum kidney function parameters, malondialdehyde level, inflammatory cytokines, and nephrotoxicity markers, while decreased certain oxidative stress indices as superoxide dismutase and glutathione refereeing to the control group ( < 0.0001). Administration of ethanol extract to CCl injured rats attenuated these changes with variable degrees. The results were confirmed through the observed amelioration of the renal histological architectures.
ethanol extract possesses potent curative effect against CCl-induced nephropathy through improvement of kidney function, oxidative stress, inflammatory and nephrotoxicity index and the renal histopathological features. To establish the therapeutic and pharmacological applications of the plant, additional researches are required.
四氯化碳（CCl）通过改变抗氧化防御系统在各种组织中诱导氧化应激。最近，植物疗法在治疗各种疾病方面的应用有了很大的增加。
本研究旨在评估（Forssk.）Benth et Hook（菊科）地上部分乙醇提取物对大鼠肾脏 CCl 诱导毒性的治疗效果。
通过腹腔注射 CCl 以 0.5 mL/kg b.wt./每周两次的剂量诱导肾毒性，连续 6 周。评估血清肾功能测试、氧化应激标志物、炎症细胞因子、肾毒性标志物和组织病理学观察。
CCl 增加了血清肾功能参数、丙二醛水平、炎症细胞因子和肾毒性标志物，而与对照组相比，某些氧化应激指标（如超氧化物歧化酶和谷胱甘肽）降低（<0.0001）。给予 CCl 损伤大鼠乙醇提取物可不同程度地减弱这些变化。通过观察到的对肾脏组织学结构的改善，证实了这些结果。
乙醇提取物通过改善肾功能、氧化应激、炎症和肾毒性指标以及肾脏组织病理学特征，对 CCl 诱导的肾病具有潜在的治疗作用。为了确定植物的治疗和药理学应用，还需要进行更多的研究。
