利用超分子 FRET 对实现脂噬可视化。
Visualization of lipophagy using a supramolecular FRET pair.
机构信息
Center for Self-assembly and Complexity (CSC), Institute for Basic Science (IBS), Pohang 37673, Republic of Korea.
Division of Advanced Materials Science, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
出版信息
Chem Commun (Camb). 2021 Nov 16;57(91):12179-12182. doi: 10.1039/d1cc04779e.
A rationally designed supramolecular FRET pair consisting of cyanine3-cucurbit[7]uril (Cy3-CB[7]) and boron-dipyrromethene 630/650-adamantylammonium (BDP-AdA) can be used to visualize organelle-specific autophagy events. The intracellular accumulations of Cy3-CB[7] in lysosomes and BDP-AdA in lipid droplets (LDs) and the formation of an intracellular host-guest complex between Cy3-CB[7] and BDP-AdA resulting in FRET signals allow us to visualize the fusion of LDs with lysosomes, namely, lipophagy. This study demonstrates the potential of supramolecular imaging based on bio-orthogonal host-guest interactions in the investigation of selective autophagy events.
一种由菁染料 3-环糊精（Cy3-CB[7]）和硼二吡咯亚甲基 630/650-金刚烷铵（BDP-AdA）组成的合理设计的超分子荧光共振能量转移（FRET）对可用于可视化细胞器特异性自噬事件。细胞内溶酶体中 Cy3-CB[7]的积累和脂滴（LDs）中 BDP-AdA 的积累以及 Cy3-CB[7]和 BDP-AdA 之间形成的细胞内主客体复合物导致 FRET 信号，使我们能够可视化 LDs 与溶酶体的融合，即脂噬作用。本研究证明了基于生物正交主客体相互作用的超分子成像在选择性自噬事件研究中的潜力。
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