López-Santacruz Hiram D, López-López Arantxa, Revilla-Guarinos Ainhoa, Camelo-Castillo Anny, Esparza-Villalpando Vicente, Mira Alex, Aranda-Romo Saray
Faculty of Dentistry, Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico.
Department of Health and Genomics, Oral Microbiome Lab, FISABIO Foundation, Avda. Cataluña 21, 46020, Valencia, Spain.
Int Microbiol. 2021 Nov;24(4):619-629. doi: 10.1007/s10123-021-00222-9. Epub 2021 Nov 3.
Streptococcus dentisani has been proposed as a promising probiotic against tooth decay, due to its ability to buffer acidic pH and to inhibit the growth of oral pathogens. However, it is unknown if this bacterial species has a global distribution. The current study aimed to establish the presence of S. dentisani in oral samples from different geographic locations by identifying the sequence of its 16S rRNA gene in available datasets from across the globe. In addition, an analytical and cross-sectional study was carried out to determine if the levels of this probiotic strain are higher in caries-free individuals compared to those with dental caries. Samples from various geographical sources demonstrated that S. dentisani is present in saliva and dental plaque from individuals of different continents. Typical S. dentisani levels in saliva ranged from 10 to 10 cells/ml and a total of 10-10 cells in dental plaque. Using real-time qPCR, S. dentisani was quantified from supragingival dental plaque of 25 caries-free and 29 caries-active individuals from a Mexican children population, where significantly higher proportions of S. dentisani were found in the caries-free group (p = 0.002). Finally, a negative correlation was found between caries levels (as measured by the dmft caries index) and the percentage of S. dentisani (p < 0.001). Thus, the current manuscript indicates that this species has a global distribution, can be found in saliva and dental plaque, and appears to be present in higher numbers in plaque samples from caries-free children.
齿双歧杆菌已被提议作为一种有前景的预防龋齿的益生菌，因为它有缓冲酸性pH值和抑制口腔病原体生长的能力。然而，这种细菌是否在全球范围内分布尚不清楚。当前的研究旨在通过在全球可用数据集中鉴定其16S rRNA基因序列，来确定不同地理位置口腔样本中齿双歧杆菌的存在情况。此外，还进行了一项分析性横断面研究，以确定与患龋齿者相比，无龋个体中这种益生菌菌株的水平是否更高。来自不同地理来源的样本表明，齿双歧杆菌存在于不同大陆个体的唾液和牙菌斑中。唾液中典型的齿双歧杆菌水平为每毫升10至10个细胞，牙菌斑中共有10至10个细胞。使用实时定量PCR，对来自墨西哥儿童群体的25名无龋个体和29名患龋个体的龈上牙菌斑中的齿双歧杆菌进行了定量，结果发现无龋组中齿双歧杆菌的比例显著更高（p = 0.002）。最后，发现龋齿水平（通过dmft龋齿指数衡量）与齿双歧杆菌百分比之间呈负相关（p < 0.001）。因此，当前的论文表明该物种在全球范围内分布，可在唾液和牙菌斑中发现，并且在无龋儿童的菌斑样本中数量似乎更高。
