Zhan Junfeng, Li Jianbo, Wu Yuerong, Wu Panfeng, Yu Ziqi, Cui Peng, Zhou Mofan, Xu Yumin, Jin Tingyu, Du Ziye, Luo Mengcheng, Liu Cong
Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China.
Department of Tissue and Embryology, School of Basic Medical Sciences, Wuhan University, Wuhan, China.
Front Vet Sci. 2021 Oct 18;8:754021. doi: 10.3389/fvets.2021.754021. eCollection 2021.
Mammalian spermatogenesis is a highly ordered process that is determined by chromatin-associated moderators which still remain poorly understood. Through a multi-control group proteomics strategy, we confirmed that Sugp2 was a chromatin-associated candidate protein, and its signal arose along spermatogenesis. The expression results showed that Sugp2, which is mainly expressed in the testis, had two transcripts, encoding one protein. During spermatogenesis, Sugp2 was enriched in the nucleus of male germ cells. With the depletion of Sugp2 by CRISPER-Cas9 technology, we found that Sugp2 controlled a network of genes on metal ion and ATP binding, suggesting that alternative splicing regulation by Sugp2 is involved in cellular ion and energy metabolism during spermatogenesis, while it had a little effect on meiotic progression and male fertility. Collectively, these data demonstrated that, as a chromatin-associated protein, Sugp2 mediated the alternative splicing regulatory network during spermatogenesis.
哺乳动物精子发生是一个高度有序的过程，由与染色质相关的调节因子决定，而这些调节因子目前仍知之甚少。通过多对照组蛋白质组学策略，我们证实Sugp2是一种与染色质相关的候选蛋白，其信号在精子发生过程中出现。表达结果表明，主要在睾丸中表达的Sugp2有两种转录本，编码一种蛋白质。在精子发生过程中，Sugp2在雄性生殖细胞核中富集。通过CRISPER-Cas9技术敲除Sugp2后，我们发现Sugp2控制着一个关于金属离子和ATP结合的基因网络，这表明Sugp2介导的可变剪接调控参与了精子发生过程中的细胞离子和能量代谢，而对减数分裂进程和雄性生育力影响较小。总的来说，这些数据表明Sugp2作为一种与染色质相关的蛋白质，在精子发生过程中介导了可变剪接调控网络。
