Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.
Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.
Biosens Bioelectron. 2022 Jan 15;196:113730. doi: 10.1016/j.bios.2021.113730. Epub 2021 Oct 29.
Alzheimer's disease (AD) is a progressive and irreversible neurodegenerative brain disorder that affects tens of millions of older adults worldwide and has significant economic and societal impacts. Despite its prevalence and severity, early diagnosis of AD remains a considerable challenge. Here we report an integrated acoustofluidics-based diagnostic system (ADx), which combines triple functions of acoustics, microfluidics, and orthogonal biosensors for clinically accurate, sensitive, and rapid detection of AD biomarkers from human plasma. We design and fabricate a surface acoustic wave-based acoustofluidic separation device to isolate and purify AD biomarkers to increase the signal-to-noise ratio. Multimodal biosensors within the integrated ADx are fabricated by in-situ patterning of the ZnO nanorod array and deposition of Ag nanoparticles onto the ZnO nanorods for surface-enhanced Raman scattering (SERS) and electrochemical immunosensors. We obtain the label-free detections of SERS and electrochemical immunoassay of clinical plasma samples from AD patients and healthy controls with high sensitivity and specificity. We believe that this efficient integration provides promising solutions for the early diagnosis of AD.
阿尔茨海默病（AD）是一种进行性且不可逆转的神经退行性脑疾病，影响着全球数千万老年人，对经济和社会造成了重大影响。尽管 AD 很常见且后果严重，但早期诊断仍然是一个巨大的挑战。在这里，我们报告了一种基于声流控的综合诊断系统（ADx），它结合了声学、微流控和正交生物传感器的三重功能，可从人血浆中临床准确、灵敏、快速地检测 AD 生物标志物。我们设计并制造了一种基于表面声波的声流控分离装置，用于分离和纯化 AD 生物标志物，以提高信噪比。集成 ADx 中的多模态生物传感器通过在 ZnO 纳米棒阵列上进行原位图案化以及在 ZnO 纳米棒上沉积 Ag 纳米颗粒来制造，用于表面增强拉曼散射（SERS）和电化学免疫传感器。我们从 AD 患者和健康对照者的临床血浆样本中获得了无标记的 SERS 和电化学免疫分析的高灵敏度和特异性检测。我们相信这种高效的集成提供了 AD 早期诊断的有前途的解决方案。
