Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
Talanta. 2022 Jan 15;237:122927. doi: 10.1016/j.talanta.2021.122927. Epub 2021 Oct 5.
Abnormal expression of microRNAs is greatly associated with the occurrence of various cancer types, revealing great potential of microRNA as biomarkers for cancer diagnosis and prognosis. Herein, a MXene-MoS heterostructure enhancing electrochemical biosensor coupled with catalytic hairpin assembly (CHA) amplification approach for label-free determination of microRNA-21 (miR-21) was successfully assembled. In particular, the unique micro-nano heterostructure with large specific area and favorable electroconductivity exhibited the ability of excellent confinement effect. Thus, rendered the MXene-MoS heterostructure the ability to trigger more target recycling reaction, giving new vitality to the traditional CHA amplification method. Meanwhile, thionine (Thi) and gold nanoparticles (AuNPs) were anchoring at the surface of MXene-MoS heterostructure, respectively, empowered the sensor the capability of capture probes fixation and miR-21 label-free determination. When numerous electronegative double-stranded DNA generated, the electron transfer was greatly hindered, resulting in signal decrease. Accordingly, the design denoted a broad dynamic range from 100 fM to 100 nM and a detection limit of about 26 fM, comparable or lower than previous reported methods for miR-21 detection. Furthermore, the sensing platform supplied satisfactory selectivity, reproducibility and stability towards the miR-21 detection. The real sample determination also showed a promising performance under clinical circumstance. Finally, from the clinical standpoint, the proposed biosensor is a considerable platform toward early disease detection and monitoring.
异常表达的 microRNAs 与各种癌症类型的发生密切相关，这揭示了 microRNA 作为癌症诊断和预后生物标志物的巨大潜力。在此，成功组装了一种 MXene-MoS 异质结构增强电化学生物传感器，该传感器结合了催化发夹组装（CHA）扩增方法，用于无标记测定 microRNA-21（miR-21）。特别是，具有大比表面积和良好导电性的独特微纳异质结构表现出优异的限域效应能力。因此，使 MXene-MoS 异质结构能够触发更多的靶标循环反应，为传统的 CHA 扩增方法注入新的活力。同时，硫堇（Thi）和金纳米粒子（AuNPs）分别锚定在 MXene-MoS 异质结构的表面上，赋予传感器固定捕获探针和无标记测定 miR-21 的能力。当产生大量带负电的双链 DNA 时，电子转移受到极大阻碍，导致信号下降。因此，该设计表示出从 100 fM 到 100 nM 的宽动态范围和约 26 fM 的检测限，与之前报道的 miR-21 检测方法相当或更低。此外，该传感平台在 miR-21 检测方面表现出令人满意的选择性、重现性和稳定性。在临床环境下，对实际样品的测定也表现出了良好的性能。最后，从临床角度来看，该生物传感器是一种用于早期疾病检测和监测的有前途的平台。
