Zhang Yajun, Meng Biao, Wei Xiao, Li Yan, Wang Xiaohui, Zheng Yan, Wang Changjun, Cui Lihong, Zhao Xiangna
Department of Gastroenterology, The Sixth Medical Center of PLA General Hospital, Beijing, People's Republic of China.
Centre for Disease Control and Prevention of China PLA, Beijing, People's Republic of China.
Infect Drug Resist. 2021 Oct 27;14:4457-4469. doi: 10.2147/IDR.S326230. eCollection 2021.
is an important nosocomial infectious bacterium, more and more multidrug resistant have been isolated and posed severe challenges to clinical antibiotic treatment, bringing additional morbidity, mortality, and economic burden. Bacteriophages can lyse bacteria specificity and are feasible alternatives to antibiotics.
A -infecting phage vB_PaeP_PA01EW was isolated. Phage plaque assays, transmission electron microscopy, host-range determination, infection assay analyses, whole-genome sequencing and annotation were performed for the phage. Mice pneumonia model using liquid aerosol-exposure was established, and phage therapy was evaluated.
vB_PaeP_PA01EW belongs to the family according to transmission electron microscopy and was identified as a according to the genome analysis. For the phage therapy, compared with the bacteria-infected group, the phage-rescue group has some characteristics. First, adventitial edema and diffuse infiltration of inflammatory cells in tissues were alleviated, Second, bronchial epithelial cell proliferation was reduced. Third, the bacterial burden was significantly decreased.
This study provided data support and theoretical basis for the clinical application of bacteriophages. It has important guiding significance and reference value for the application of bacteriophage therapy of other pathogenic bacteria.
是一种重要的医院感染病原菌，越来越多的多重耐药菌株被分离出来，给临床抗生素治疗带来严峻挑战，增加了发病率、死亡率和经济负担。噬菌体能够特异性裂解细菌，是抗生素可行的替代物。
分离出一株感染的噬菌体vB_PaeP_PA01EW。对该噬菌体进行噬菌斑测定、透射电子显微镜观察、宿主范围测定、感染试验分析、全基因组测序和注释。建立了液体气溶胶暴露的小鼠肺炎模型，并对噬菌体治疗进行评估。
根据透射电子显微镜观察，vB_PaeP_PA01EW属于科，根据基因组分析被鉴定为。对于噬菌体治疗，与细菌感染组相比，噬菌体挽救组有一些特点。第一，组织中外膜水肿和炎性细胞弥漫性浸润减轻；第二，支气管上皮细胞增殖减少；第三，细菌载量显著降低。
本研究为噬菌体的临床应用提供了数据支持和理论依据，对其他病原菌的噬菌体治疗应用具有重要的指导意义和参考价值。
