Ren Xinxin, Wang Xiang, Peng Bi, Liang Qiuju, Cai Yuan, Gao Kewa, Hu Yongbin, Xu Zhijie, Yan Yuanliang
Center for Molecular Medicine, Xiangya Hospital, Central South University, Changsha, People's Republic of China.
Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, People's Republic of China.
Int J Gen Med. 2021 Oct 27;14:7133-7143. doi: 10.2147/IJGM.S336602. eCollection 2021.
To explore the molecular profiles of transcriptional enhanced associate domain () family in ovarian serous carcinoma (OSC).
In this study, we use bioinformatics methods including GEPIA, GE-mini, Oncomine 3.0, Kaplan-Meier plotter, cBioPortal, WebGestalt, TIMER2.0 and DiseaseMeth2.0, and in vitro experimental RT-PCR to assess the expression profiles and prognostic significance of family in OSC.
According to the bioinformatics analysis, family was abnormally expressed in OSC. In terms of prognosis, Kaplan-Meier plotter indicated that OSC patients with high level of TEAD4 showed poor overall survival (OS), progression-free survival (PFS) and post progression survival (PPS). family also had significantly diagnostic values for OSC patients. Tumor Immune Estimation Resource (TIMER) algorithm indicated that family was significantly associated with different types of infiltrating immune cells, including B cells, macrophages, dendritic cells, neutrophils, CD8+ T cells and CD4+ T cells. Gene set enrichment analysis of family-associated coexpression genes was further explored. In in vitro experiments, the RT-PCR results showed the upregulated TEAD2/4 in OSC tissues and cells (A2780 and TOV112D). Moreover, decreased expression of TEAD2 could induce the ferroptosis through increasing the ROS accumulation.
Thus, family correlated with the diagnosis, prognosis and immune infiltration in OSC. These results could provide comprehensive understanding of family in the diagnosis and prognosis of OSC patients.
探讨转录增强关联结构域（TEAD）家族在卵巢浆液性癌（OSC）中的分子特征。
在本研究中，我们使用包括GEPIA、GE-mini、Oncomine 3.0、Kaplan-Meier Plotter、cBioPortal、WebGestalt、TIMER2.0和DiseaseMeth2.0在内的生物信息学方法，以及体外实验性逆转录聚合酶链反应（RT-PCR）来评估TEAD家族在OSC中的表达谱和预后意义。
根据生物信息学分析，TEAD家族在OSC中异常表达。在预后方面，Kaplan-Meier Plotter表明TEAD4水平高的OSC患者总生存期（OS）、无进展生存期（PFS）和进展后生存期（PPS）较差。TEAD家族对OSC患者也具有显著的诊断价值。肿瘤免疫估计资源（TIMER）算法表明，TEAD家族与不同类型的浸润免疫细胞显著相关，包括B细胞、巨噬细胞、树突状细胞、中性粒细胞、CD8 + T细胞和CD4 + T细胞。进一步探索了TEAD家族相关共表达基因的基因集富集分析。在体外实验中，RT-PCR结果显示OSC组织和细胞（A2780和TOV112D）中TEAD2/4上调。此外，TEAD2表达降低可通过增加活性氧（ROS）积累诱导铁死亡。
因此，TEAD家族与OSC的诊断、预后和免疫浸润相关。这些结果可为全面了解TEAD家族在OSC患者诊断和预后中的作用提供依据。
