Subramanyam Gunashekar Kalvakunta, Gaddam Susmila Aparna, Kotakadi Venkata Subbaiah, Palithya Sashikiran, Penchalaneni Josthna, Challagundla Varadarajulu Naidu
Department of Biotechnology, Dravidian University, Kuppam, India.
Department of Virology, Sri Venkateswara University, Tirupati, India.
Artif Cells Nanomed Biotechnol. 2021 Dec;49(1):635-650. doi: 10.1080/21691401.2021.1996384.
In the present investigation, green synthesis of silver nanoparticles (AgNPs) was carried out using aqueous leaf extract of . The results of the spectral characterisation have revealed that the surface Plasmon resonance band was observed at 421 nm confirms the formation of AgNPs. The Fourier Transform Infra-red Spectroscopy result shows the reduction of silver nitrate into AgNPs by the reduction of different functional groups. Transmission Electron Microscope analysis revealed that the particles are roughly spherical and poly-disperse in shape and size, the particles are within the size range of 10-55 nm. Dynamic Light Scattering revealed that the nanoparticles were also within the range of 10-50 nm, An-AgNPs have a high negative zeta potential value of -38.9 mV. An-AgNPs showed efficient free radical scavenging activity and showed excellent antimicrobial activity. Anti-proliferative and cytotoxic effect of An-AgNPs was carried out by MTT assay against KB oral cancer cells, the IC value of An-AgNPs is 58.64 µg/ml. The cell's growth is arrested at the G2/M phase, so the An-AgNPs activated the Caspase 3 pathway which leads to the Apoptosis of KB oral cancer cells. So it is concluded that the green synthesised have manifold functions.
在本研究中，使用[植物名称]的水叶提取物进行了银纳米颗粒（AgNPs）的绿色合成。光谱表征结果表明，在421nm处观察到表面等离子体共振带，证实了AgNPs的形成。傅里叶变换红外光谱结果表明，通过不同官能团的还原作用，硝酸银被还原为AgNPs。透射电子显微镜分析显示，颗粒形状大致为球形，尺寸多分散，粒径在10 - 55nm范围内。动态光散射表明，纳米颗粒也在10 - 50nm范围内，An - AgNPs具有-38.9mV的高负zeta电位值。An - AgNPs表现出高效的自由基清除活性，并具有优异的抗菌活性。通过MTT法对KB口腔癌细胞进行An - AgNPs的抗增殖和细胞毒性作用研究，An - AgNPs的IC值为58.64μg/ml。细胞生长停滞在G2/M期，因此An - AgNPs激活了半胱天冬酶3途径，导致KB口腔癌细胞凋亡。所以得出结论，绿色合成的[物质名称]具有多种功能。
