Truong Thi Thao, Khieu Thi Tam, Luu Huu Nguyen, Truong Hai Bang, Nguyen Van Khien, Vuong Truong Xuan, Tran Thi Kim Ngan
Faculty of Chemistry, TNU-University of Sciences, Tan Thinh Ward, Thai Nguyen City 250000, Vietnam.
Laboratory of Magnetism and Magnetic Materials, Science and Technology Advanced Institute, Van Lang University, Ho Chi Minh City 700000, Vietnam.
Materials (Basel). 2023 Aug 3;16(15):5457. doi: 10.3390/ma16155457.
Green synthesis and nanomaterials have been the current trends in biomedical materials. In this study, L. leaf extract-doped ZnO nanoparticles (PLE-doped ZnO NPs), a novel nanomaterial, were studied including the synthesis process, and the biomedical activity was evaluated. PLE-doped ZnO NPs were synthesized by the co-precipitation method, with differences in the synthesis procedures and dosages of the extract. The X-ray diffraction, Fourier transform infrared, scanning electron microscopy, energy dispersive X-ray spectroscopy, Brunauer-Emmett-Teller, ultraviolet-visible diffuse reflectance spectroscopy, and photoluminescence spectrum analysis results showed that the biosynthesized PLE-doped ZnO NPs were pure and in a hexagonal wurtzite phase. The PLE-doped NPs were synthesized by adding the extract to the zinc acetate solution before adjusting the pH and exhibited the smallest size (ZPS50 was 22 nm), the richest in the surface organic functional groups and the best optical activity. The highest antibacterial activity against and was observed at 100 µg/mL of ZPS50 NPs, and the inhibition zone reached 42 and 39 nm, respectively. Moreover, ZPS50 NPs showed a moderate effectiveness against KB cancer cells with an IC value of 43.53 ± 2.98 µg/mL. This present study's results suggested that ZPS50 NPs could be a promising nanomaterial in developing drugs for treating human epithelial carcinoma cells and infectious illnesses.
绿色合成与纳米材料一直是生物医学材料领域的当前趋势。在本研究中，对一种新型纳米材料——L.叶提取物掺杂的ZnO纳米颗粒（PLE掺杂的ZnO NPs）进行了研究，包括其合成过程，并评估了其生物医学活性。PLE掺杂的ZnO NPs通过共沉淀法合成，提取物的合成程序和用量有所不同。X射线衍射、傅里叶变换红外光谱、扫描电子显微镜、能量色散X射线光谱、布鲁诺尔-埃米特-泰勒比表面积测定法、紫外可见漫反射光谱和光致发光光谱分析结果表明，生物合成的PLE掺杂的ZnO NPs是纯净的，呈六方纤锌矿相。PLE掺杂的纳米颗粒是通过在调节pH值之前将提取物添加到醋酸锌溶液中合成的，其尺寸最小（ZPS50为22 nm），表面有机官能团最丰富，光学活性最佳。在ZPS50纳米颗粒浓度为100 µg/mL时，观察到对[具体菌种1]和[具体菌种2]的最高抗菌活性，抑菌圈分别达到42和39 nm。此外，ZPS50纳米颗粒对KB癌细胞显示出中等疗效，IC值为43.53±2.98 µg/mL。本研究结果表明，ZPS50纳米颗粒可能是开发治疗人类上皮癌细胞和传染病药物的一种有前景的纳米材料。
