gen. nov., sp. nov., a novel member of the family isolated from toxin-producing dinoflagellate .

A Gram-stain-negative, aerobic, rod-shaped strain (R2A-3) was isolated from the toxin-producing dinoflagellate and identified as a novel genus and new species based on a polyphasic taxonomic approach. The optimum conditions for growth of the strain were at 25 °C, pH 8.0 and in the presence of 3 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA genes and 92 core genes sets revealed that strain R2A-3 belongs to the family in the class and represented an independent taxon separated from other genera. The 16S rRNA gene of strain R2A-3 showed the highest sequence similarity to TG408 (95.2%), HA-01 (94.1%) and NH6-24 (93.2%), and less than 92.8 % similarity to other genera in the family . The genome length of strain R2A-3 was 3608892 bp with 65.2 mol% G+C content. Summed feature 8 (comprising C 7 and/or C 6) was the major fatty acid (>10 %). Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were detected as the major polar lipids. The major respiratory quinone was ubiquinone-8. According to its phylogenetic, phenotypic, chemotaxonomic and genomic features, strain R2A-3 represents a new species in the new genus of the family . It is recommended to name it gen. nov., sp. nov. The type strain is R2A-3 (=KCTC 82469=GDMCC 1.2523).