College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
Swine Research Institute, Tie Qi Li Shi Group Co., Mianyang, 621006, China.
Biol Trace Elem Res. 2022 Sep;200(9):3975-3982. doi: 10.1007/s12011-021-03009-1. Epub 2021 Nov 5.
Intrauterine growth retardation (IUGR) is the main death cause of newborn piglets in large-scale farms. To investigate the effects of maternal nano-selenium (nano-Se) and Macleaya cordata extracts (MCE) on immune functions of IUGR piglets in large scale farms, a 2 × 2 factorial design was adopted in this test, and two factors were nano-Se (0, 0.50 mg/kg) and MCE (0, 500 mg/kg). A total of 32 ternary hybrid sows (Landrace × Yorkshire × Duroc, parity 2) were used in this 25-day trial from day 90 of pregnancy to delivery. The dietary treatments were as follows: (1) CON group, basic diet (0.0 mg/kg Se); (2) Nano-Se group, basic diet + 0.50 mg/kg added Se (nano-Se); (3) MCE group, basic diet + 500 mg/kg added MCE; (4) Combined group, basic diet + 0.50 mg/kg added nano-Se and 500 mg/kg added MCE. Maternal nano-Se or combination of nano-Se and MCE diets extremely increased the superoxide dismutase (SOD), catalase (CAT), superoxide dismutase (GSH-Px) contents in the serum and liver of IUGR offspring (P < 0.05), and MCE supplementation in sow diets remarkably increased the serum superoxide dismutase (SOD), catalase (CAT), and superoxide dismutase (GSH-Px) contents of IUGR piglets (P < 0.05). Adding nano-Se, MCE, or nano-Se and MCE to sow diets decreased the malondialdehyde (MDA) content in the serum and liver of IUGR piglets (P < 0.05). The supplementation of nano-Se and combined diets extremely increased the activities of immunoglobulin G (IgG) and immunoglobulin A (IgA) in the serum and liver of IUGR offspring (P < 0.05). Maternal nano-Se, MCE, and combined diets greatly decreased the levels of tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), and interleukin-1β (IL-1β) in the serum and liver of IUGR piglets (P < 0.05). Together, the application of nano-Se and/or MCE to sow diets improved antioxidant capacities and immune functions of IUGR offspring, and alleviated oxidative stress.
宫内生长迟缓（IUGR）是规模化养殖场新生仔猪的主要死亡原因。为了研究母源纳米硒（nano-Se）和博落回提取物（MCE）对规模化养殖场 IUGR 仔猪免疫功能的影响，本试验采用 2×2 析因设计，设 2 个因素，即纳米硒（0、0.50mg/kg）和 MCE（0、500mg/kg）。试验选用 32 头三元杂交母猪（长白猪×约克夏猪×杜洛克猪，胎次 2），从妊娠第 90 天到分娩进行了为期 25 天的试验。日粮处理如下：（1）CON 组，基础日粮（0.0mg/kg Se）；（2）纳米硒组，基础日粮+0.50mg/kg 添加硒（纳米硒）；（3）MCE 组，基础日粮+500mg/kg 添加 MCE；（4）联合组，基础日粮+0.50mg/kg 添加纳米硒和 500mg/kg 添加 MCE。母源纳米硒或纳米硒和 MCE 日粮的组合极显著提高了 IUGR 后代血清和肝脏中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）的含量（P<0.05），MCE 添加到母猪日粮中极显著提高了 IUGR 仔猪的血清超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的含量（P<0.05）。添加纳米硒、MCE 或纳米硒和 MCE 到母猪日粮中降低了 IUGR 仔猪血清和肝脏中丙二醛（MDA）的含量（P<0.05）。纳米硒和联合日粮的补充极显著提高了 IUGR 后代血清和肝脏中免疫球蛋白 G（IgG）和免疫球蛋白 A（IgA）的活性（P<0.05）。母源纳米硒、MCE 和联合日粮极显著降低了 IUGR 仔猪血清和肝脏中肿瘤坏死因子-α（TNF-α）、白细胞介素 6（IL-6）和白细胞介素 1β（IL-1β）的水平（P<0.05）。总之，纳米硒和/或 MCE 添加到母猪日粮中提高了 IUGR 后代的抗氧化能力和免疫功能，并缓解了氧化应激。
