Department of Medicine, Division of Hematology and Medical Oncology, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Mount Sinai Institute for Systems Biomedicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Department of Cancer Immunology and Virology, Dana Farber Cancer Institute, Boston, MA, USA.
Stem Cell Reports. 2021 Dec 14;16(12):3036-3049. doi: 10.1016/j.stemcr.2021.10.005. Epub 2021 Nov 4.
A library of well-characterized human induced pluripotent stem cell (hiPSC) lines from clinically healthy human subjects could serve as a useful resource of normal controls for in vitro human development, disease modeling, genotype-phenotype association studies, and drug response evaluation. We report generation and extensive characterization of a gender-balanced, racially/ethnically diverse library of hiPSC lines from 40 clinically healthy human individuals who range in age from 22 to 61 years. The hiPSCs match the karyotype and short tandem repeat identities of their parental fibroblasts, and have a transcription profile characteristic of pluripotent stem cells. We provide whole-genome sequencing data for one hiPSC clone from each individual, genomic ancestry determination, and analysis of mendelian disease genes and risks. We document similar transcriptomic profiles, single-cell RNA-sequencing-derived cell clusters, and physiology of cardiomyocytes differentiated from multiple independent hiPSC lines. This extensive characterization makes this hiPSC library a valuable resource for many studies on human biology.
一个由临床健康的人类供体来源的经过充分特征鉴定的人诱导多能干细胞（hiPSC）细胞库，可以作为体外人类发育、疾病建模、基因型-表型关联研究和药物反应评估的正常对照的有用资源。我们报告了一个性别平衡、种族/民族多样化的 hiPSC 细胞库的产生和广泛特征鉴定，该细胞库来自 40 名临床健康的个体，年龄从 22 岁到 61 岁不等。hiPSCs 与它们的成纤维细胞的核型和短串联重复序列身份相匹配，并且具有多能干细胞特征的转录谱。我们提供了每个人中一个 hiPSC 克隆的全基因组测序数据、基因组祖先的确定，以及孟德尔疾病基因和风险的分析。我们记录了相似的转录组谱、单细胞 RNA 测序衍生的细胞簇，以及从多个独立的 hiPSC 系分化而来的心肌细胞的生理学。这种广泛的特征鉴定使这个 hiPSC 细胞库成为许多人类生物学研究的宝贵资源。
