Department of System Science, Kobe University Graduate School of System Informatics, Kobe, Japan.
Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Neurosci Res. 2022 Jun;179:57-64. doi: 10.1016/j.neures.2021.10.012. Epub 2021 Nov 2.
Holographic structured illumination combined with optogenetics enables patterned stimulation of neurons and glial cells in an intact living brain. Moreover, in vivo functional imaging of cellular activity with recent advanced microscope technologies allows for visualization of the cellular responses during learning, emotion and cognition. Integrating these techniques can be used to verify the link between cell function and behavior output. However, there are technical limitations to stimulate multiple cells with high spatial and temporal resolution with available techniques of optogenetic stimulation. Here, we summarized a two-photon microscope combined with holographic system to stimulate multiple cells with high spatial and temporal resolution for living mice and their biological application.
全息结构光照结合光遗传学能够对完整活体大脑中的神经元和神经胶质细胞进行模式化刺激。此外，利用最近先进的显微镜技术对细胞活动进行体内功能成像，可在学习、情绪和认知过程中观察细胞反应。整合这些技术可用于验证细胞功能与行为输出之间的联系。然而，现有的光遗传学刺激技术在高时空分辨率刺激多个细胞方面存在技术限制。在这里，我们总结了一种双光子显微镜与全息系统相结合的方法，用于对活体小鼠的多个细胞进行高时空分辨率刺激及其生物学应用。
