Wang Qian, Wei Zhenyu, Zhu Haijing, Pan Chuanying, Akhatayeva Zhanerke, Song Xiaoyue, Lan Xianyong
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, China.
Animals (Basel). 2023 Jun 18;13(12):2023. doi: 10.3390/ani13122023.
The () gene, as the major gene responsible for growth, plays a vital role in myogenesis. Meanwhile, the relationship between copy number variation (CNV) of this gene and growth traits in goats remains unclear. Therefore, this study investigated four aspects: bioinformatics analysis, mRNA expression (n = 6), CNV detection (n = 224), and association analysis. The findings indicated that the gene had a large number of conserved motifs, and the gene expression level was higher in fetal goats than in adult goats. Three CNV loci were selected from the database, among which CNV1 was located in the bidirectional promoter region and was associated with goat growth traits. CNV analysis showed that CNV2 and CNV3 of the gene were associated with growth traits such as body weight, heart girth, height at hip cross, and hip width ( < 0.05), with CNV1 loss genotype being the superior genotype, and CNV2 and CNV3 median and gain genotypes of being superior genotypes. This finding further confirms that the gene is the dominant gene for growth traits, which will serve as theoretical guidance for goat breeding.
（）基因作为负责生长的主要基因，在肌肉生成中起着至关重要的作用。同时，该基因的拷贝数变异（CNV）与山羊生长性状之间的关系仍不清楚。因此，本研究从四个方面进行了调查：生物信息学分析、mRNA表达（n = 6）、CNV检测（n = 224）和关联分析。研究结果表明，该基因有大量保守基序，且该基因在胎山羊中的表达水平高于成年山羊。从数据库中筛选出三个CNV位点，其中CNV1位于双向启动子区域，与山羊生长性状相关。CNV分析表明，该基因的CNV2和CNV3与体重、胸围、臀端高和臀宽等生长性状相关（P < 0.05），CNV1缺失基因型为优势基因型，CNV2和CNV3的中位数和增益基因型为优势基因型。这一发现进一步证实该基因是生长性状的主导基因，将为山羊育种提供理论指导。
