Department of Chemistry, Kansas State University, Manhattan, KS, USA.
Center for Biomedical Engineering, University of Texas Medical Branch, Galveston, TX, USA.
Nanomedicine. 2022 Feb;40:102476. doi: 10.1016/j.nano.2021.102476. Epub 2021 Nov 4.
We report the design and adaptation of iron/iron oxide nanoparticle-based optical nanobiosensors for enzymes or cytokine/chemokines that are established biomarkers of lung diseases. These biomarkers comprise ADAM33, granzyme B, MMP-8, neutrophil elastase, arginase, chemokine (C-C motif) ligand 20 and interleukin-6. The synthesis of nanobiosensors for these seven biomarkers, their calibration with commercially available enzymes and cytokines/chemokines, as well as their validation using bronchoalveolar lavage (BAL) obtained from a mouse model of TLR3-mediated inflammation are discussed here. Exhaled Breath Condensate (EBC) is a minimally invasive approach for sampling airway fluid in the diagnosis and management of various lung diseases in humans (e.g., asthma, COPD and viral infections). We report the proof-of-concept of using human EBC in conjunction with nanobiosensors for diagnosis/monitoring airway inflammation. These findings suggest that, with nanosensor technology, human EBC can be utilized as a liquid biopsy to monitor inflammation/remodeling in lung disease.
我们报告了基于铁/氧化铁纳米颗粒的光学纳米生物传感器的设计和适应性，用于检测作为肺部疾病生物标志物的酶或细胞因子/趋化因子。这些生物标志物包括 ADAM33、颗粒酶 B、MMP-8、中性粒细胞弹性蛋白酶、精氨酸酶、趋化因子（C-C 基序）配体 20 和白细胞介素 6。本文讨论了这些七种生物标志物的纳米生物传感器的合成、与市售酶和细胞因子/趋化因子的校准，以及使用 TLR3 介导的炎症小鼠模型获得的支气管肺泡灌洗液 (BAL) 的验证。呼出气冷凝物 (EBC) 是一种微创方法，可用于在人类中诊断和管理各种肺部疾病时采样气道液（例如，哮喘、COPD 和病毒感染）。我们报告了使用人类 EBC 结合纳米生物传感器进行诊断/监测气道炎症的概念验证。这些发现表明，通过纳米传感器技术，人类 EBC 可用于作为液体活检来监测肺部疾病中的炎症/重塑。
