Bautista Daniel, Guayazan-Palacios Natalia, Buitrago Maria Camila, Cardenas Martha, Botero David, Duitama Jorge, Bernal Adriana J, Restrepo Silvia
Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.
Department of Biology, University of Washington, Seattle, WA, United States.
Front Plant Sci. 2021 Oct 21;12:730251. doi: 10.3389/fpls.2021.730251. eCollection 2021.
is a tree from the Andean region bearing edible fruits, considered an exotic export. Although there has been renewed interest in its commercialization, sustainability, and disease management have been limiting factors. is a recently described species that causes late blight in . There is no general study of the response of , particularly, in the changes in expression of pathogenesis-related genes. In this manuscript we present a comprehensive RNA-seq time-series study of the plant response to the infection of . Following six time points of infection, the differentially expressed genes (DEGs) involved in the defense by the plant were contextualized in a sequential manner. We documented 5,628 DEGs across all time-points. From 6 to 24 h post-inoculation, we highlighted DEGs involved in the recognition of the pathogen by the likely activation of pattern-triggered immunity (PTI) genes. We also describe the possible effect of the pathogen effectors in the host during the effector-triggered response. Finally, we reveal genes related to the susceptible outcome of the interaction caused by the onset of necrotrophy and the sharp transcriptional changes as a response to the pathogen. This is the first report of the transcriptome of the tree tomato in response to the newly described pathogen
是一种来自安第斯地区的树，结出可食用果实，被视为一种外来出口产品。尽管人们对其商业化重新产生了兴趣，但可持续性和病害管理一直是限制因素。是一种最近被描述的物种，会在中引起晚疫病。目前尚无关于对，特别是对病程相关基因表达变化的反应的全面研究。在本手稿中，我们展示了一项关于植物对感染反应的全面RNA测序时间序列研究。在感染的六个时间点之后，植物防御中涉及的差异表达基因（DEGs）以连续的方式进行了情境化分析。我们记录了所有时间点的5628个DEGs。在接种后6至24小时，我们突出了可能通过模式触发免疫（PTI）基因的激活参与病原体识别的DEGs。我们还描述了病原体效应子在效应子触发反应期间在宿主中的可能作用。最后，我们揭示了与坏死营养开始导致的相互作用的易感结果以及作为对病原体反应的急剧转录变化相关的基因。这是树番茄转录组对新描述病原体反应的首次报告
