Ren Wei, Chen Li, Wang Qian, Ren Yanping
State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
Fukang Station of Desert Ecology, Chinese Academy of Sciences, Fukang 831505, China.
Life (Basel). 2022 Jun 20;12(6):921. doi: 10.3390/life12060921.
Upland cotton () is a salt-tolerant crop that can withstand high salinity levels without showing signs of harm to the plant. However, the plant is more prone to salinity stress at the germination stage and a poor germination as well as poor crop stand lead to a weak productivity. It is possible to obtain a comprehensive picture of the cotton seedling germination and establishment against salt stress by examining dynamic changes in the transcriptomic and metabolomic profiles. The reported study employed a pretreatment of cotton seeds by soaking them in 0.2% Magnesium Sulphate (MgSO) solution at room temperature for 4, 8, and 12 h. The analysis of variance based on the studied traits emergence rate, above and underground plant parts' fresh weight measured, displayed significant differences of the three treatments compared with the control. A total of 28,801 and 264 differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were discovered to code for biological processes such as response to salt stress, cellular response to salt stress, abscisic acid receptor PYR/PYL, regulation of seed growth and germination, and auxin-activated signaling pathways. A large amount of ethylene-responsive transcription factors (ERF) was identified (1235) as differentially expressed, followed by (252), (96), (202), (81), (64), (28), and -box (26) in treated seedling samples. Functional enrichment analysis revealed the significant roles in the hormones and signal transduction, carbohydrates metabolism, and biosynthesis of amino acids, promoting salt stress tolerance. Our results indicated positive effects of MgSO at 4 h treatment on seedling germination and growth, seemingly by activating certain growth-regulating enzymes (auxins, gibberellins, jasmonates, abscisic acid, and salicylic acid) and metabolites (phenolic acids, flavonoids, and akaloids). Such pretreatment of MgSO on seeds would be beneficial in future cotton management under saline conditions to enhance good crop stand and productivity.
陆地棉（）是一种耐盐作物，能够承受高盐度水平而不表现出对植株的伤害迹象。然而，该植物在萌发阶段更容易受到盐胁迫，发芽不良以及作物生长势差会导致生产力低下。通过检查转录组和代谢组谱的动态变化，可以全面了解棉花幼苗在盐胁迫下的萌发和生长情况。所报道的研究采用了在室温下将棉花种子浸泡在0.2%硫酸镁（MgSO）溶液中4、8和12小时的预处理方法。基于所研究的性状（出苗率、地上和地下植株部分鲜重）进行的方差分析显示，与对照相比，这三种处理存在显著差异。共发现28801个差异表达基因（DEGs）和264个差异积累代谢物（DAMs），它们编码诸如对盐胁迫的响应、细胞对盐胁迫的响应、脱落酸受体PYR/PYL、种子生长和萌发的调控以及生长素激活信号通路等生物学过程。在处理后的幼苗样本中，大量乙烯响应转录因子（ERF）被鉴定为差异表达（1235个），其次是（252个）、（96个）、（202个）、（81个）、（64个）、（28个）和-box（26个）。功能富集分析揭示了它们在激素和信号转导、碳水化合物代谢以及氨基酸生物合成中发挥的重要作用，从而促进盐胁迫耐受性。我们的结果表明，4小时的硫酸镁处理对幼苗萌发和生长有积极影响，似乎是通过激活某些生长调节酶（生长素、赤霉素、茉莉酸、脱落酸和水杨酸）和代谢物（酚酸、黄酮类化合物和生物碱）实现的。这种对种子的硫酸镁预处理在未来盐渍条件下的棉花管理中有利于提高作物生长势和生产力。
