Wang Bo, Zhu Shuoji, Deng Yuzhi, Sai Xiyalatu, Chen Zerui, Liu Jian, Li Ge, Liu Nanbo, Chen Jimei, Yu Changjiang, Sun Tucheng, Zhu Ping
Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China.
Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China; The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, China; Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao City, Inner Mongolia, Peoples Republic China.
Stem Cell Res. 2021 Dec;57:102590. doi: 10.1016/j.scr.2021.102590. Epub 2021 Nov 2.
As a subunit of the nucleosome remodeling and histone deacetylation (NuRD) complex, GATA zinc finger domain containing 2B (GATAD2B) plays a vital role in chromatin modification and transcriptional regulation. To further investigate the role of GATAD2B in cell fate determination of human ESCs, we generated two GATAD2B homozygous knockout human ESC lines by CRISPR/Cas9 technology. The cell line exhibits normal karyotype and typical stem cell morphology, following the high expression of pluripotent genes and differentiation potential in vitro. These cell lines will provide cell resources to investigate epigenetic regulation in extensive biological processes as menthioned above.
作为核小体重塑和组蛋白去乙酰化（NuRD）复合物的一个亚基，含GATA锌指结构域2B（GATAD2B）在染色质修饰和转录调控中起着至关重要的作用。为了进一步研究GATAD2B在人类胚胎干细胞（hESC）细胞命运决定中的作用，我们利用CRISPR/Cas9技术构建了两个GATAD2B纯合敲除的hESC系。该细胞系具有正常的核型和典型的干细胞形态，同时在体外具有多能基因的高表达和分化潜能。这些细胞系将为研究上述广泛生物学过程中的表观遗传调控提供细胞资源。
