Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, School of Life Sciences, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Tianjin University, Tianjin 300072, China.
School of Medical Imaging, Xuzhou Medical University, Xuzhou 221006, Jiangsu, China.
ACS Biomater Sci Eng. 2021 Dec 13;7(12):5717-5726. doi: 10.1021/acsbiomaterials.1c01216. Epub 2021 Nov 10.
With the development of technology, adjuvant immunotherapy has become a promising strategy for prevention of postoperative tumor regression and metastasis by stimulating the host immune response. However, the therapeutic effects are still unsatisfactory due to the lack of synergy between different methods. In this study, an efficient synergistic immunotherapy system based on injectable sodium alginate hydrogels was designed to inhibit recurrence and metastasis at the same time. On the one hand, an injectable sodium alginate (SA) hydrogel microsystem loaded with toll-like receptor (TLR) agonists (CpG ODNs) was synthesized for inhibiting recurrence, and then carcinoembryonic antigen (CEA) probe was also added to detect CEA based on fluorescence resonance energy transfer (FRET) technology to monitor the occurrence and development of tumor recurrence. On the other hand, an anti-programmed cell death 1 ligand 1 antibody (anti-PD-L1)-modified SA nanogel loaded with indocyanine green (ICG@SA-anti-PD-L1 nanogel) was prepared for diagnosing and inhibiting lung metastasis by assisting orthotopic tumor therapy. and results demonstrated that this SA micro/nanosystem could monitor and inhibit postoperative recurrence and metastasis. We hope that this micro/nano-synergistic system will become an effective strategy for postoperative adjuvant immunotherapy.
随着技术的发展，通过刺激宿主免疫反应，辅助免疫疗法已成为预防术后肿瘤复发和转移的一种有前途的策略。然而，由于不同方法之间缺乏协同作用，治疗效果仍不尽人意。在这项研究中，设计了一种基于可注射海藻酸钠水凝胶的高效协同免疫治疗系统，以同时抑制复发和转移。一方面，合成了一种载有 Toll 样受体（TLR）激动剂（CpG ODNs）的可注射海藻酸钠（SA）水凝胶微系统，用于抑制复发，然后还添加了癌胚抗原（CEA）探针，基于荧光共振能量转移（FRET）技术来检测 CEA，以监测肿瘤复发的发生和发展。另一方面，制备了载有吲哚菁绿（ICG@SA-anti-PD-L1 纳米凝胶）的抗程序性细胞死亡配体 1 抗体（anti-PD-L1）修饰的 SA 纳米凝胶，通过辅助原位肿瘤治疗来诊断和抑制肺转移。结果表明，该 SA 微/纳米系统可监测和抑制术后复发和转移。我们希望这种微/纳米协同系统将成为术后辅助免疫治疗的有效策略。
