Comparative evaluation of antimicrobial and antifungal efficacy of bioactive root-end filling materials: An study.

CONTEXT

Microorganisms are the main cause of failure of endodontic treatment. When retreatment fails periapical surgery followed by retrograde filling is done to seal the apex. A root-end filling material should have antimicrobial property as well as bioactive properties necessary for healing, repair, and regeneration of the apex.

AIMS

The aim of this study was to evaluate the antibacterial and antifungal efficacy of three bioactive root-end filling materials: mineral trioxide aggregate (MTA) Plus, Biodentine, Endosequence root repair material (ERRM) against and .

SUBJECTS AND METHODS

and standard bacterial strains were used. 100 μl was taken from liquid cultures of and planted in Mueller-Hinton agar and the same amount of was planted in Sabouraud dextrose agar by lawn culture. MTA Plus, Biodentine, and ERRM were aseptically filled into the opened pits. Following this, the media were kept in the drying oven at 37°C for 24, 48, and 72 h and the diameters of the inhibition zones were measured.

STATISTICAL ANALYSIS USED

Statistical analysis was carried out by Kruskal-Wallis, (Mann-Whitney), Friedman, and (Wilcoxon-sign) test.

RESULTS

Among the three groups, the antimicrobial activity of Biodentine against was statistically higher than MTA Plus and ERRM ( < 0.05). Antifungal activity of MTA Plus against was statistically higher than Biodentine and ERRM ( < 0.05). ERRM showed the smallest inhibition zone against and among the three groups ( < 0.05).

CONCLUSIONS

Biodentine exhibited the greatest antimicrobial activity and MTA Plus exhibited the greatest antifungal activity among the three groups. ERRM exhibited the least antibacterial and antifungal activity among the three groups.