使用试管稀释法评估两种新材料的最低抑菌浓度：一项体外研究。
Evaluation of minimal inhibitory concentration of two new materials using tube dilution method: An in vitro study.
作者信息
Hiremath Geeta S, Kulkarni Raghavendra D, Naik Balaram D
机构信息
Department of Conservative Dentistry and Endodontics, Sri Dharmasthala Manjunatheshwara College of Medical Sciences and Hospital, Dharwad, Karnataka, India.
Department of Microbiology, Sri Dharmasthala Manjunatheshwara College of Medical Sciences and Hospital, Dharwad, Karnataka, India.
出版信息
J Conserv Dent. 2015 Mar-Apr;18(2):159-62. doi: 10.4103/0972-0707.153056.
AIM
The aim of the study is to evaluate and compare the antimicrobial efficacy of two new materials MTA Plus and Biodentine with ProRoot MTA using tube dilution method.
MATERIALS AND METHODS
The materials used were ProRoot MTA (Dentsply), MTA Plus (compounded by Prevest Denpro, Jammu, India for Avalon Biomed Inc, USA) and a calcium silicate based material Biodentine (Septodont, Saint-Maur-des-Fosses, France). Doubling dilutions of the material were prepared in Sabouraud's dextrose broth (SDB) and Brain Heart Infusion (BHI) broth for Candida albicans and Enterococcus faecalis, respectively. The minimal concentration at which inhibition of microorganism occurred was measured and noted as minimal inhibitory concentration (MIC) of the material.
RESULTS
There was no statistically significant difference between the materials against C. albicans. Biodentine was statistically significant than MTA Plus against E. faecalis (P-value-0.022). ProRoot MTA was statistically significant at different time intervals against E. faecalis (P-value-0.001).
CONCLUSION
ProRoot MTA and Biodentine proved to have antimicrobial property. MTA Plusproved as a good antifungal agent.
目的
本研究旨在采用试管稀释法评估并比较两种新材料MTA Plus和Biodentine与ProRoot MTA的抗菌效果。
材料与方法
所用材料为ProRoot MTA（登士柏公司）、MTA Plus（由印度查谟的Prevest Denpro公司为美国阿瓦隆生物医学公司配制）和一种硅酸钙基材料Biodentine（法国圣莫尔-德福塞的Septodont公司）。分别在沙氏葡萄糖肉汤（SDB）和脑心浸液（BHI）肉汤中对材料进行双倍稀释，用于白色念珠菌和粪肠球菌的培养。测量并记录抑制微生物生长的最低浓度，作为该材料的最低抑菌浓度（MIC）。
结果
各材料对白色念珠菌的抗菌效果无统计学显著差异。Biodentine对粪肠球菌的抗菌效果在统计学上显著优于MTA Plus（P值 = 0.022）。ProRoot MTA在不同时间间隔对粪肠球菌的抗菌效果具有统计学显著性（P值 = 0.001）。
结论
ProRoot MTA和Biodentine具有抗菌性能。MTA Plus被证明是一种良好的抗真菌剂。
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