Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000, Niš, Serbia.
Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000, Niš, Serbia.
Food Chem Toxicol. 2021 Dec;158:112666. doi: 10.1016/j.fct.2021.112666. Epub 2021 Nov 9.
Immortelle (Helichrysum italicum, Asteraceae) essential oil has been widely used in alternative medicine to accelerate wound healing, as well as in cosmetic products to stimulate skin regeneration and to reduce the appearance of wrinkles. It is also considered a natural and safe culinary spice that could also be applied in the food industry as a preservative in the future. The therapeutic efficacy of this oil changes with the natural variability of the composition. Herein we tested and mutually compared the antimicrobial and anti-inflammatory activities of four commercial immortelle oils differing in the relative amounts of marker compounds, i.e. neryl esters, α-pinene, γ- and ar-curcumenes, and β-diketones. The anti-inflammatory effect of selected chromatographic fractions, enriched in the aforementioned constituents, was evaluated by studying toxicity toward rat peritoneal macrophages, their nitric oxide production, myeloperoxidase, and arginase activities. Subsequently, the compositional and activity data were subjected to a multivariate statistical treatment to reveal the possible correlation(s) between the percentage of essential-oil constituents and the observed activities. The obtained results imply that immortelle oil efficiency as an antimicrobial and/or anti-inflammatory agent is most plausibly a result of a synergistic action between its constituents, and/or, rather unexpectedly, the presence of some minor constituents.
蜡菊（Helichrysum italicum，菊科）精油在替代医学中被广泛用于加速伤口愈合，在化妆品中用于刺激皮肤再生和减少皱纹。它也被认为是一种天然且安全的烹饪香料，将来也可以在食品工业中用作防腐剂。这种油的治疗功效随其组成的自然可变性而变化。在此，我们测试并相互比较了四种商业蜡菊油的抗菌和抗炎活性，这四种油在标记化合物（即香叶基酯、α-蒎烯、γ-和 α-姜黄烯、以及β-二酮）的相对含量上有所不同。通过研究对大鼠腹腔巨噬细胞的毒性、一氧化氮产生、髓过氧化物酶和精氨酸酶活性，评估了富含上述成分的选定色谱馏分的抗炎作用。随后，对成分和活性数据进行了多元统计处理，以揭示精油成分的百分比与观察到的活性之间的可能相关性。所得结果表明，蜡菊油作为抗菌和/或抗炎剂的功效很可能是其成分协同作用的结果，或者出人意料的是，某些少量成分的存在。
