Department of Biomolecular Sciences, Plant Biology Section, University of Urbino Carlo Bo, Urbino, Italy.
Department of Pharmacy, University of Pisa, Pisa, Italy.
J Med Food. 2019 Oct;22(10):1041-1046. doi: 10.1089/jmf.2019.0054. Epub 2019 Jun 14.
The chemical composition of the essential oil of flowering aerial parts of subsp. cultivated in central Italy, Marche region, was analyzed by means of gas chromatography-mass spectrometry. Seventy-eight components, accounting for 98.71% of the whole essential oil composition, were identified and quantified. Neryl acetate showed the largest relative abundance in the composition, accounting for 15.75% of the oil, followed by -pinene (8.21%); 4,6,9-trimethyl-8-decene-3,5-dione, (Italidione I), (7.34%); -curcumene and -selinene (5.37%); -curcumene (4.83%); nerol (4.75%); -selinene (4.68%); limonene (4.55%); linalool (4.42%), and 2,4,6,9-tetramethyl-8-decene-3,5-dione (Italidione II), (4.26%). The oil inhibited collagenase and elastase activities, with IC values of 36.99 ± 1.52 and 135.43 ± 6.32 g/mL, respectively. Neryl acetate, nerol, and linalool, distinctive compounds of the oil obtained from this plant, tested alone or in mixture, at the same percentages shown in the essential oil, exhibited no activity against the two enzymes. On the contrary, -pinene and limonene, tested alone and in mixture, showed inhibitory activity on both collagenase and elastase.
采用气相色谱-质谱联用技术分析了生长在意大利中部马尔凯地区的 亚种开花地上部分精油的化学成分。共鉴定出 78 种成分，占精油组成的 98.71%。乙酸橙花酯在组成中具有最大的相对丰度，占油的 15.75%，其次是 -蒎烯（8.21%）；4,6,9-三甲基-8-癸烯-3,5-二酮，（意大利二酮 I），（7.34%）；-姜黄烯和 -蛇床烯（5.37%）；-姜黄烯（4.83%）；橙花醇（4.75%）；-蛇床烯（4.68%）；柠檬烯（4.55%）；芳樟醇（4.42%）和 2,4,6,9-四甲基-8-癸烯-3,5-二酮（意大利二酮 II），（4.26%）。该油抑制胶原蛋白酶和弹性蛋白酶的活性，IC 值分别为 36.99±1.52 和 135.43±6.32μg/mL。从这种植物中获得的油中的独特化合物橙花基乙酸酯、橙花醇和芳樟醇，单独或混合使用，以与精油中相同的百分比测试，对两种酶均无活性。相反，-蒎烯和柠檬烯单独和混合使用时，对胶原蛋白酶和弹性蛋白酶均显示出抑制活性。
