Department of Biosciences, Durham University, Durham, DH1 3LE, UK; Department of Chemistry, Durham University, Durham, DH1 3LE, UK.
Department of Biosciences, Durham University, Durham, DH1 3LE, UK; Department of Chemistry, Durham University, Durham, DH1 3LE, UK.
Curr Opin Chem Biol. 2022 Feb;66:102095. doi: 10.1016/j.cbpa.2021.102095. Epub 2021 Nov 8.
Inorganic metals supplement the chemical repertoire of organic molecules, especially proteins. This requires the correct metals to associate with proteins at metalation. Protein mismetalation typically occurs when excesses of unbound metals compete for a binding site ex vivo. However, in biology, excesses of metal-binding sites typically compete for limiting amounts of exchangeable metals. Here, we summarise mechanisms of metal homeostasis that sustain optimal metal availabilities in biology. We describe recent progress to understand metalation by comparing the strength of metal binding to a protein versus the strength of binding to competing sites inside cells.
无机金属补充了有机分子（尤其是蛋白质）的化学组成。这需要正确的金属与蛋白质在金属化过程中结合。蛋白质非配位金属化通常发生在过量的未结合金属竞争结合位点时，这种情况发生在体外。然而，在生物学中，过量的金属结合位点通常会竞争有限数量的可交换金属。在这里，我们总结了维持生物学中最佳金属可用性的金属稳态机制。我们通过比较金属与蛋白质结合的强度与细胞内竞争结合位点的结合强度，来描述理解金属化的最新进展。
