Aleixandre C, Miller D A, Mitchell A R, Warburton D A, Gersen S L, Disteche C, Miller O J
Hum Genet. 1987 Sep;77(1):46-50. doi: 10.1007/BF00284712.
A cloned alphoid sequence, p82H, hybridizes in situ to the centromere of every human chromosome. After washing under stringent conditions, no more than 8% of the grains are located on any specific chromosome. p82H thus differs from other centromeric sequences which are reported to be chromosome specific, because it detects sequences that are conserved among the chromosomes. Two experimental approaches show that the p82H sequences are closely associated with the centromere. First, p82H remains with the relocated centromeres in an inv(19) and an inv(6) chromosome. Second, p82H hybridizes at the centromere but not to the centromeric heterochromatin of chromosomes 1, 9 and 16 that have elongated 1qh, 9qh and 16qh regions produced by short growth in 5-azacytidine. The only noncentromeric site of hybridization is at the distal end of the 9qh region.
一个克隆的α卫星序列p82H，可原位杂交到每个人类染色体的着丝粒上。在严格条件下洗涤后，位于任何特定染色体上的银粒不超过8%。因此，p82H与其他据报道具有染色体特异性的着丝粒序列不同，因为它能检测到染色体间保守的序列。两种实验方法表明，p82H序列与着丝粒紧密相关。第一，在一个inv(19)和一个inv(6)染色体中，p82H与重新定位的着丝粒在一起。第二，p82H在着丝粒处杂交，但不与1号、9号和16号染色体的着丝粒异染色质杂交，这些染色体由于在5-氮杂胞苷中短时间生长而产生了延长的1qh、9qh和16qh区域。唯一的非着丝粒杂交位点在9qh区域的远端。
