Daba Abera, Berecha Gezahegn, Tadesse Mekuria, Belay Addisie
Department of Horticulture and Plant Science, College of Agriculture and Veterinary Medicine, Jimma University, Jimma, Ethiopia.
Department of Horticulture, Faculty of Agriculture, Wollega University, Nekemte, Ethiopia.
Saudi J Biol Sci. 2021 Nov;28(11):6408-6416. doi: 10.1016/j.sjbs.2021.07.011. Epub 2021 Jul 10.
Weeds are the most productive limiting factor, especially in organic farming systems where the uses of synthetic herbicides are not allowed due to their negative impacts. Hence, synthetic herbicides need to be replaced with biological herbicides for weed management. Thus, the present study was designed to evaluate the herbicidal activity of conidia suspensions from , , , , , and against weed via a series of laboratory and lath-house conditions that laid out in a CRD and RCBD, respectively, with three replications for each bioassay. The results revealed that all fungi, except , had significantly reduced seed germination as well as early growth of the target weed compared to the untreated control. The inhibitory effects were measured to be varied among the types of conidia suspensions of fungal species and their level of concentration. The highest rate of inhibition was observed for conidia suspension from which suppressed with the maximum seed germination inhibitory level (65%) over control. Likewise, the plumule and radicle growth length of the target weed also significantly inhibited by the tested fungi (ranging from 10 to 85% and 34 to 97%) compared to the control, respectively. Based on their efficacy in the laboratory bioassay, the herbicidal potential of selected fungi was further evaluated in pot experiments. In contrarily to laboratory observations, the effect of different fungal conidia suspensions on various growth parameters of the targeted weed was insignificant in the lath-house experiments. In conclusion, the application of displayed some potential green light to be investigated as a biocontrol agent with promising retarding in the germination and early growth of . Hence, we recommend further investigation of those fungi under field conditions on different coffee weed species.
杂草是最具生产力的限制因素，尤其是在有机耕作系统中，由于合成除草剂的负面影响，不允许使用它们。因此，需要用生物除草剂替代合成除草剂来进行杂草管理。因此，本研究旨在通过一系列分别以完全随机设计（CRD）和随机区组设计（RCBD）布置的实验室和温室条件，评估来自[多个真菌名称未给出]的分生孢子悬浮液对[目标杂草名称未给出]杂草的除草活性，每个生物测定重复三次。结果表明，除了[某个真菌名称未给出]之外，与未处理的对照相比，所有真菌都显著降低了目标杂草的种子萌发以及早期生长。测量发现，真菌种类的分生孢子悬浮液类型及其浓度水平之间的抑制作用各不相同。观察到来自[某个真菌名称未给出]的分生孢子悬浮液抑制率最高，其对种子萌发的抑制水平比对照高65%。同样，与对照相比，测试真菌也显著抑制了目标杂草的胚芽和胚根生长长度（分别为10%至85%和34%至97%）。基于它们在实验室生物测定中的功效，在盆栽试验中进一步评估了所选真菌的除草潜力。与实验室观察结果相反，在温室试验中，不同真菌分生孢子悬浮液对目标杂草各种生长参数的影响不显著。总之，[某个真菌名称未给出]的应用显示出一些潜在的可能性，可作为一种生物防治剂进行研究，有望延缓[目标杂草名称未给出]的萌发和早期生长。因此，我们建议在田间条件下对不同咖啡杂草种类的这些真菌进行进一步研究。
